9VUL

Cryo-EM structure of the human measles virus RNA-dependent RNA polymerase bound to allosteric inhibitor ERDRP-0519

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Maltose/maltodextrin-binding periplasmic protein,Phosphoprotein	polymer	602	66334.4	4	UniProt (P0AEX9) UniProt (P35974)	Escherichia coli K-12	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Protein P
2	E (L)	Maltose/maltodextrin-binding periplasmic protein,RNA-directed RNA polymerase L,Strep II and FLAG tag	polymer	2630	297006.9	1	UniProt (P0AEX9) UniProt (P35975)	Escherichia coli K-12	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Protein L,Large structural protein,Replicase,Transcriptase
3	F, G (L)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)
4	H (L)	2-methyl-~{N}-[4-[(2~{S})-2-(2-morpholin-4-ylethyl)piperidin-1-yl]sulfonylphenyl]-5-(trifluoromethyl)pyrazole-3-carboxamide	non-polymer		529.6	1	Chemie (A1EF9) PubChem (57521469)

Sequence modifications

A, B, C, D: -80 - 283 (UniProt: P0AEX9)

PDB	External Database	Details
Met -94	-	initiating methionine
Gly -93	-	expression tag
Ser -92	-	expression tag
Ser -91	-	expression tag
His -90	-	expression tag
His -89	-	expression tag
His -88	-	expression tag
His -87	-	expression tag
His -86	-	expression tag
His -85	-	expression tag
Gly -84	-	expression tag
Thr -83	-	expression tag
Lys -82	-	expression tag
Thr -81	-	expression tag
Gly 284	-	linker
Thr 285	-	linker
Asp 286	-	linker
Tyr 287	-	linker
Asp 288	-	linker
Ile 289	-	linker
Pro 290	-	linker
Thr 291	-	linker
Thr 292	-	linker
Leu 293	-	linker
Glu 294	-	linker
Val 295	-	linker
Leu 296	-	linker
Phe 297	-	linker
Gln 298	-	linker
Gly 299	-	linker
Pro 300	-	linker
Leu 301	-	linker
Gly 302	-	linker
Ser 303	-	linker

A, B, C, D: 304 - 507 (UniProt: P35974)
L: -383 - -20 (UniProt: P0AEX9)

PDB	External Database	Details
Met -397	-	initiating methionine
Gly -396	-	expression tag
Ser -395	-	expression tag
Ser -394	-	expression tag
His -393	-	expression tag
His -392	-	expression tag
His -391	-	expression tag
His -390	-	expression tag
His -389	-	expression tag
His -388	-	expression tag
Gly -387	-	expression tag
Thr -386	-	expression tag
Lys -385	-	expression tag
Thr -384	-	expression tag
Gly -19	-	linker
Thr -18	-	linker
Asp -17	-	linker
Tyr -16	-	linker
Asp -15	-	linker
Ile -14	-	linker
Pro -13	-	linker
Thr -12	-	linker
Thr -11	-	linker
Leu -10	-	linker
Glu -9	-	linker
Val -8	-	linker
Leu -7	-	linker
Phe -6	-	linker
Gln -5	-	linker
Gly -4	-	linker
Pro -3	-	linker
Leu -2	-	linker
Gly -1	-	linker
Ser 0	-	linker

L: 1 - 2183 (UniProt: P35975)
L: 2184 - 2232 (PDB: 9VUL)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	562344.4
Non-Polymers*	Number of molecules	3
	Total formula weight	660.4
All*	Total formula weight	563004.8

*Water molecules are not included.