9VOS
Cryo-EM Structure of GPR158 in Complex with RGS7-Gbeta5 and Nanobody Nb20
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (B, A) | Metabotropic glycine receptor | polymer | 781 | 88251.6 | 2 | UniProt (Q5T848) Pfam (PF22572) Pfam (PF00003) | Homo sapiens (human) | mGlyR,G-protein coupled receptor 158 |
| 2 | C (C) | Isoform 2 of Regulator of G-protein signaling 7 | polymer | 469 | 54761.9 | 1 | UniProt (P49802) Pfam (PF00610) Pfam (PF18148) Pfam (PF00631) Pfam (PF00615) | Homo sapiens (human) | RGS7 |
| 3 | D (D) | Guanine nucleotide-binding protein subunit beta-5 | polymer | 353 | 38778.6 | 1 | UniProt (P62881) Pfam (PF25391) | Mus musculus (house mouse) | Gbeta5,Transducin beta chain 5 |
| 4 | E, F (H, I) | Nb20 | polymer | 150 | 16722.5 | 2 | Lama glama | ||
| 5 | AA, BA, G, H, I... (A, B) | CHOLESTEROL | non-polymer | 386.7 | 22 | Chemie (CLR) PubChem (5997) | |||
| 6 | CA (A) | 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine | non-polymer | 744.0 | 1 | Chemie (PEE) | |||
| 7 | DA (A) | 2-[(HYDROXY{[(2R,3R,5S,6R)-2,3,4,5,6-PENTAHYDROXYCYCLOHEXYL]OXY}PHOSPHORYL)OXY]-1-[(PALMITOYLOXY)METHYL]ETHYL HEPTADECANOATE | non-polymer | 825.1 | 1 | Chemie (PII) |
Sequence modifications
B, A: 1 - 775 (UniProt: Q5T848)
| PDB | External Database | Details |
|---|---|---|
| Leu 776 | - | expression tag |
| Glu 777 | - | expression tag |
| Val 778 | - | expression tag |
| Leu 779 | - | expression tag |
| Phe 780 | - | expression tag |
| Gln 781 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 303488.7 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 10075.5 | |
| All* | Total formula weight | 313564.1 |
