9V9T

Cryo-EM structure of the ncPRC1.6 complex bound to the H2AK119ubH2BK120ub-modified nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (L, N)	RING1 and YY1-binding protein	polymer	228	24863.6	2	UniProt (Q8N488)	Homo sapiens (human)	Apoptin-associating protein 1,APAP-1,Death effector domain-associated factor,DED-associated factor,YY1 and E4TF1-associated factor 1
2	C, G (A, E)	Histone H3	polymer	136	15435.1	2	UniProt (A0A310TTQ1)	Xenopus laevis (African clawed frog)
3	D, H (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62799)	Xenopus laevis (African clawed frog)
4	E, I (C, G)	Histone H2A	polymer	130	14083.4	2	UniProt (Q6AZJ8)	Xenopus laevis (African clawed frog)
5	F, J (D, H)	Histone H2B	polymer	126	13913.2	2	UniProt (A0A8J0TWI5)	Xenopus laevis (African clawed frog)
6	K, L (K, O)	Ubiquitin	polymer	76	8622.9	2	UniProt (P0CG47)	Homo sapiens (human)
7	M (I)	DNA (131-MER)	polymer	131	40237.6	1		Homo sapiens
8	N (J)	DNA (132-MER)	polymer	132	40942.1	1		Homo sapiens
9	O, P (L, N)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)

Sequence modifications

C, G: 0 - 129 (UniProt: Q6AZJ8)

PDB	External Database	Details
Cys 119	Lys 120	engineered mutation

D, H: -3 - 122 (UniProt: A0A8J0TWI5)

PDB	External Database	Details
Cys 117	Lys 121	engineered mutation

K, O: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Cys 76	Gly 228	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	257805.1
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	257935.9

*Water molecules are not included.