9V1H

Cryo-EM structure of Gi-coupled NPFF2R in complex with GUB08248

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha-1	polymer	361	41641.2	1		Homo sapiens
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	371	40227.0	1	UniProt (P62873)	Homo sapiens (human)
3	C (E)	scFv16	polymer	247	26277.3	1		Homo sapiens
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
5	E (L)	GUB08248	polymer	11	1234.5	1		Homo sapiens
6	F (R)	Neuropeptide FF receptor 2	polymer	420	48734.1	1	UniProt (Q9Y5X5)	Homo sapiens (human)	G-protein coupled receptor 74,G-protein coupled receptor HLWAR77,Neuropeptide G-protein coupled receptor

Sequence modifications

B: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag
Gly 346	-	expression tag
Ser 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Gly 357	-	expression tag
Ser 358	-	expression tag
Ser 359	-	expression tag
Gly 360	-	expression tag
Val 361	-	expression tag
Ser 362	-	expression tag
Gly 363	-	expression tag
Trp 364	-	expression tag
Arg 365	-	expression tag
Leu 366	-	expression tag
Phe 367	-	expression tag
Lys 368	-	expression tag
Lys 369	-	expression tag
Ile 370	-	expression tag
Ser 371	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	165844.0
All*	Total formula weight	165844.0

*Water molecules are not included.