9V10
Cryo-EM structure of the 48-nm repeat doublet microtubule from mouse sperm
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, H (0, 7) | Cilia- and flagella-associated protein 95 | polymer | 228 | 26633.0 | 2 | UniProt (Q9CQC3) Pfam (PF15139) | Mus musculus (house mouse) | |
| 2 | B, C (1, 2) | EF-hand domain-containing family member B | polymer | 853 | 95892.0 | 2 | UniProt (Q8CDU5) Pfam (PF13499) Pfam (PF25325) | Mus musculus (house mouse) | Cilia- and flagella-associated protein 21 |
| 3 | D, E (3, 4) | Cilia- and flagella-associated protein 53 | polymer | 514 | 62036.6 | 2 | UniProt (Q9D439) Pfam (PF13868) | Mus musculus (house mouse) | Coiled-coil domain-containing protein 11 |
| 4 | F, FV, G (5, 6, j1) | Nucleoside diphosphate kinase homolog 7 | polymer | 395 | 44489.8 | 3 | UniProt (Q9QXL8) Pfam (PF25364) Pfam (PF00334) | Mus musculus (house mouse) | NDK 7,NDP kinase homolog 7,3'-5' exonuclease NME7,Protein kinase NME7,nm23-H7 |
| 5 | I (8) | Cilia- and flagella-associated protein 107 | polymer | 196 | 23062.5 | 1 | UniProt (Q4KKZ1) Pfam (PF22595) | Mus musculus (house mouse) | |
| 6 | J, PJ (A, N1) | Cilia- and flagella-associated protein 141 | polymer | 101 | 12278.1 | 2 | UniProt (Q9D9D9) Pfam (PF15104) | Mus musculus (house mouse) | |
| 7 | K, L, M, N, O... (A0, A1, A2, A3, A4...) | Tektin-1 | polymer | 418 | 48713.0 | 6 | UniProt (Q9DAJ2) Pfam (PF03148) | Mus musculus (house mouse) | |
| 8 | AA, AE, AF, AG, AI... (AA, AC, AE, AG, AI...) | Tubulin alpha-3 chain | polymer | 450 | 50012.4 | 167 | UniProt (P05214) Pfam (PF00091) Pfam (PF03953) | Mus musculus (house mouse) | Alpha-tubulin 3/7,Alpha-tubulin isotype M-alpha-3/7,Tubulin alpha-3/alpha-7 chain |
| 9 | AB, AC, AD, AH, AJ... (AB, AD, AF, AH, AJ...) | Tubulin beta-4B chain | polymer | 445 | 49877.8 | 170 | UniProt (P68372) Pfam (PF00091) Pfam (PF03953) | Mus musculus (house mouse) | Tubulin beta-2C chain |
| 10 | GA, HA, IA (Aa, Ab, Ac) | Sperm axonemal maintenance protein CFAP97D1 | polymer | 164 | 19811.6 | 3 | UniProt (Q9DAN9) Pfam (PF13879) | Mus musculus (house mouse) | CFAP97 domain-containing protein 1 |
| 11 | IB, JA (B, C) | Meiosis-specific nuclear structural protein 1 | polymer | 491 | 60353.0 | 2 | UniProt (Q61884) Pfam (PF13868) | Mus musculus (house mouse) | |
| 12 | KA, LA, MA, MN, NA... (B0, B1, B2, B3, B4...) | Tektin-2 | polymer | 430 | 50385.1 | 12 | UniProt (Q922G7) Pfam (PF03148) | Mus musculus (house mouse) | Tektin-t,Testicular tektin |
| 13 | FB, GB, HB (Ba, Bb, Bc) | EF-hand calcium-binding domain-containing protein 3 | polymer | 432 | 49795.1 | 3 | UniProt (Q80X60) Pfam (PF13833) | Mus musculus (house mouse) | |
| 14 | HO, IO, JB, JO, KB... (C0, C1, C2, C3, C4...) | Tektin-3 | polymer | 490 | 56754.8 | 16 | UniProt (Q6X6Z7) Pfam (PF03148) | Mus musculus (house mouse) | |
| 15 | DC, FH (D, K3) | Sperm-associated antigen 8 | polymer | 470 | 50591.8 | 2 | UniProt (Q3V0Q6) Pfam (PF22584) | Mus musculus (house mouse) | Sperm membrane protein 1,SMP-1,Sperm membrane protein BS-84 |
| 16 | EC, FC, GC, GP, HC... (D0, D1, D2, D3, D4...) | Tektin-4 | polymer | 447 | 52121.4 | 12 | UniProt (Q149S1) Pfam (PF03148) | Mus musculus (house mouse) | Testicular microtubules-related protein 4 |
| 17 | OD, QJ, YC (E, F, N2) | Cilia- and flagella-associated protein 161 | polymer | 303 | 34433.4 | 3 | UniProt (Q6P8Y0) Pfam (PF24569) | Mus musculus (house mouse) | |
| 18 | ND (Ea) | Ciliary microtubule inner protein 1 | polymer | 136 | 15610.0 | 1 | UniProt (Q9DA42) Pfam (PF14945) | Mus musculus (house mouse) | Uncharacterized protein C20orf85 homolog |
| 19 | CE, DE, EE, FE (Fa, Fb, Fc, Fd) | Ciliary microtubule inner protein 3 | polymer | 210 | 23717.5 | 4 | UniProt (Q8K168) Pfam (PF22581) | Mus musculus (house mouse) | |
| 20 | GE (G) | Piercer of microtubule wall 2 protein | polymer | 122 | 13728.5 | 1 | UniProt (V9GXK1) Pfam (PF14892) | Mus musculus (house mouse) | |
| 21 | VE (Ga) | Ciliary microtubule inner protein 4 | polymer | 266 | 30278.8 | 1 | UniProt (Q9D9J2) Pfam (PF15400) | Mus musculus (house mouse) | Testis-expressed protein 33 |
| 22 | AU, BM, BU, CH, CM... (H, I, J, K, L...) | Ciliary microtubule inner protein 2A | polymer | 319 | 36659.6 | 16 | UniProt (Q9D4K5) Pfam (PF10629) | Mus musculus (house mouse) | |
| 23 | LF, MF (Ha, Hb) | Luteinizing hormone beta | polymer | 310 | 34424.4 | 2 | UniProt (A0A571BEL8) | Mus musculus (house mouse) | |
| 24 | OF (I1) | Cilia- and flagella-associated protein 68 | polymer | 166 | 19552.7 | 1 | UniProt (Q9D131) Pfam (PF06608) | Mus musculus (house mouse) | |
| 25 | EG, FG, GG (Ia, Ib, Ic) | Protein SPMIP1 | polymer | 177 | 20439.8 | 3 | UniProt (B9EJX3) Pfam (PF22589) | Mus musculus (house mouse) | ATP6V1F neighbor gene protein homolog,Protein ATP6V1FNB,Sperm-associated microtubule inner protein 1 |
| 26 | IG, JG, KG, LG, MG (J1, J2, J3, J4, J5) | Cilia- and flagella-associated protein 77 | polymer | 283 | 32786.0 | 5 | UniProt (A0A087WRI3) Pfam (PF14825) | Mus musculus (house mouse) | |
| 27 | DH, EH (K1, K2) | Cilia- and flagella-associated protein 144 | polymer | 135 | 16155.2 | 2 | UniProt (Q5NC57) Pfam (PF14886) | Mus musculus (house mouse) | Testis highly expressed protein 6 |
| 28 | WH, XH (Ka, Kb) | Protein SPMIP3 | polymer | 145 | 17001.3 | 2 | UniProt (Q9DAA7) Pfam (PF17670) | Mus musculus (house mouse) | Sperm microtubule inner protein 3,Sperm-associated microtubule inner protein 3 |
| 29 | ZH (L1) | Cilia- and flagella-associated protein 90 | polymer | 182 | 20886.4 | 1 | UniProt (Q9DAR0) Pfam (PF15074) | Mus musculus (house mouse) | Y regulated sperm protein |
| 30 | QI, RI (La, Lb) | Sperm-associated microtubule inner protein 4 | polymer | 588 | 67755.7 | 2 | UniProt (Q9D5Y0) Pfam (PF15093) | Mus musculus (house mouse) | Protein TISP74 |
| 31 | TI, UI, VI, WI (M1, M2, M3, M4) | Ciliary microtubule inner protein 2C | polymer | 200 | 23098.0 | 4 | UniProt (Q9DAS2) Pfam (PF10629) | Mus musculus (house mouse) | |
| 32 | NJ (Ma) | Protein SPMIP7 | polymer | 441 | 50476.3 | 1 | UniProt (Q5NC83) Pfam (PF15073) | Mus musculus (house mouse) | Post-meiotic spermatogenesis protein 1,Sperm microtubule inner protein 7,Spermatogenesis-associated protein 48 |
| 33 | HK, IK, JK, KK, LK (Na, Nb, Nc, Nd, Ne) | Sperm microtubule inner protein 8 | polymer | 216 | 24985.7 | 5 | UniProt (Q6IMH0) Pfam (PF22574) | Mus musculus (house mouse) | Testis,prostate and placenta-expressed protein |
| 34 | MK, SM, TL (O, P, Q) | RIB43A-like with coiled-coils protein 1 | polymer | 379 | 45032.2 | 3 | UniProt (Q9D0B8) Pfam (PF05914) | Mus musculus (house mouse) | |
| 35 | NK, OK, PK, QK (O1, O2, O3, O4) | Dual specificity phosphatase 21 | polymer | 189 | 21527.6 | 4 | UniProt (Q9D9D8) Pfam (PF00782) | Mus musculus (house mouse) | |
| 36 | AQ, FP, RK, SK, ZQ (O5, O6, T, U, V) | EF-hand domain-containing protein 1 | polymer | 648 | 75235.4 | 5 | UniProt (Q9D9T8) Pfam (PF06565) Pfam (PF13499) | Mus musculus (house mouse) | Myoclonin-1 |
| 37 | LL, ML, NL, OL, PL... (Oa, Ob, Oc, Od, Oe...) | Sperm microtubule inner protein 6 | polymer | 260 | 29977.2 | 8 | UniProt (Q2MH31) Pfam (PF15181) | Mus musculus (house mouse) | Ciliated bronchial epithelial protein 1,Spermatid-specific manchette-related protein 1,Testis development protein NYD-SP22 |
| 38 | UL, VL (P1, P2) | Sperm microtubule inner protein 11 | polymer | 131 | 15528.6 | 2 | UniProt (Q8CDT5) Pfam (PF22593) | Mus musculus (house mouse) | Testis-expressed protein 49 |
| 39 | AM, WL, XL, YL, ZL (P3, P4, P5, P6, P7) | Protein SPMIP9 | polymer | 180 | 21069.1 | 5 | UniProt (Q9DAG4) Pfam (PF15217) | Mus musculus (house mouse) | Sperm microtubule inner protein 9,Testis-expressed sequence 37 protein,Testis-specific conserved protein of 21 kDa |
| 40 | TM, UM, VM, WM (Q1, Q2, Q3, Q4) | Tektin bundle-interacting protein 1 | polymer | 206 | 23987.3 | 4 | UniProt (A6H6Q4) Pfam (PF15041) | Mus musculus (house mouse) | |
| 41 | GO, LN (R, S) | RIB43A-like with coiled-coils protein 2 | polymer | 377 | 45328.2 | 2 | UniProt (Q9D4Q1) Pfam (PF05914) | Mus musculus (house mouse) | |
| 42 | AR, AS, BQ, BR, BS... (U0, U1, U2, U3, U4...) | Tektin-5 | polymer | 557 | 62817.5 | 26 | UniProt (G5E8A8) Pfam (PF03148) | Mus musculus (house mouse) | |
| 43 | HT, US, YT, ZR (W, X, Y, Z) | EF-hand domain-containing family member C2 | polymer | 750 | 87758.0 | 4 | UniProt (Q9D485) Pfam (PF06565) | Mus musculus (house mouse) | |
| 44 | VS, WS (X4, X5) | Stabilizer of axonemal microtubules 4 | polymer | 427 | 47873.1 | 2 | UniProt (Q148A4) Pfam (PF15691) | Mus musculus (house mouse) | Protein phosphatase 1 regulatory subunit 32 |
| 45 | XS, YS, ZS (X6, X7, X8) | Testis-specific serine/threonine-protein kinase 6 | polymer | 273 | 30332.9 | 3 | UniProt (Q925K9) Pfam (PF00069) | Mus musculus (house mouse) | TSK-6,TSSK-6,Testis-specific kinase 6,Serine/threonine-protein kinase SSTK,Small serine/threonine kinase |
| 46 | AT, BT, CT, DT, ET... (XB, XC, XD, XE, XF...) | Cilia- and flagella-associated protein 20 | polymer | 193 | 22781.4 | 7 | UniProt (Q8BTU1) Pfam (PF05018) | Mus musculus (house mouse) | Gene trap locus 3 protein |
| 47 | IT, JT, KT, LT, MT... (Y0, Y1, Y2, Y3, Y4...) | Ciliary microtubule associated protein 1A | polymer | 254 | 27691.0 | 8 | UniProt (Q920N1) Pfam (PF07004) | Mus musculus (house mouse) | Outer dense fiber of sperm tails protein 3,Outer dense fiber protein 3,Sperm tail protein SHIPPO 1 |
| 48 | QT, RT, ST, TT, UT... (YA, YB, YC, YD, YE...) | Parkin coregulated gene protein homolog | polymer | 241 | 27763.3 | 8 | UniProt (Q9DAK2) Pfam (PF10274) | Mus musculus (house mouse) | Hypertension-related protein 1-like protein,PARK2 coregulated gene protein |
| 49 | DU, IU, JU, PU, QU (a, a6, b, c, d) | Cilia- and flagella-associated protein 45 | polymer | 551 | 65962.0 | 5 | UniProt (Q9D9U9) Pfam (PF13868) | Mus musculus (house mouse) | Coiled-coil domain-containing protein 19,Nasopharyngeal epithelium-specific protein 1 |
| 50 | EU, FU, GU, HU (a1, a2, a3, a4) | Sperm-associated microtubule inner protein 5 | polymer | 229 | 25847.5 | 4 | UniProt (Q9CQT6) Pfam (PF22573) | Mus musculus (house mouse) | |
| 51 | KU, LU, MU, NU, OU (b1, b2, b3, b4, b5) | Tektin-like protein 1 | polymer | 499 | 57406.5 | 5 | UniProt (Q9D4K7) Pfam (PF03148) | Mus musculus (house mouse) | Coiled-coil domain-containing protein 105 |
| 52 | RU, SU, TU (e, f, g) | Cilia- and flagella-associated protein 52 | polymer | 620 | 68322.2 | 3 | UniProt (Q5F201) Pfam (PF00400) | Mus musculus (house mouse) | WD repeat-containing protein 16 |
| 53 | EV, GV, UU, ZU (h, i, j, k) | Enkurin | polymer | 255 | 29587.5 | 4 | UniProt (Q6SP97) Pfam (PF13864) | Mus musculus (house mouse) | |
| 54 | VU, WU, XU, YU (h1, h2, h3, h4) | Sperm-associated microtubule inner protein 10 | polymer | 141 | 16305.6 | 4 | UniProt (Q9D9I1) Pfam (PF14983) | Mus musculus (house mouse) | Testis-expressed protein 43 |
| 55 | AV, HV, IV, JV (i1, l, m, n) | Protein Flattop | polymer | 189 | 20575.1 | 4 | UniProt (Q6P8X9) Pfam (PF22611) | Mus musculus (house mouse) | Cilia- and flagella-associated protein 126 |
| 56 | BV, CV, DV (i2, i3, i4) | EF-hand calcium-binding domain-containing protein 6 | polymer | 1516 | 176028.3 | 3 | UniProt (Q6P1E8) Pfam (PF13202) Pfam (PF13833) Pfam (PF08976) Pfam (PF13499) | Mus musculus (house mouse) | DJ-1-binding protein,DJBP |
| 57 | KV, LV (o, p) | Cilia- and flagella- associated protein 210 | polymer | 547 | 65266.5 | 2 | UniProt (A0JLY1) Pfam (PF13868) | Mus musculus (house mouse) | Coiled-coil domain-containing protein 173 |
| 58 | MV, NV, OV (q, r, s) | Cilia- and flagella-associated protein 276 | polymer | 168 | 18960.1 | 3 | UniProt (Q9DAD0) Pfam (PF12494) | Mus musculus (house mouse) | |
| 59 | PV (y) | Piercer of microtubule wall 1 protein | polymer | 167 | 18862.9 | 1 | UniProt (Q5BN45) Pfam (PF14892) | Mus musculus (house mouse) | Pierce1,p53-induced expression in RB-null cells protein 1 |
| 60 | QV (ke) | Sperm microtubule inner protein 2 | polymer | 189 | 22372.7 | 1 | UniProt (E9QAV2) Pfam (PF15123) | Mus musculus (house mouse) | |
| 61 | AAA, ABA, AGA, AIA, AJA... (FE, GI, ME, OI, PK...) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 167 | Chemie (GTP) | |||
| 62 | ACA, AFA, AMA, ANA, APA... (HL, LC, TE, UH, WO...) | MAGNESIUM ION | non-polymer | 24.3 | 170 | Chemie (MG) PubChem (888) | |||
| 63 | ADA, AEA, AHA, ALA, AOA... (IP, KB, NF, SB, VL...) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 170 | Chemie (GDP) | |||
| 64 | BPA, DPA, FPA (X6, X7, X8) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 3 | Chemie (ATP) |
Sequence modifications
5, 6, j1: 1 - 395 (UniProt: Q9QXL8)
| PDB | External Database | Details |
|---|---|---|
| Val 50 | Thr 50 | conflict |
| Asn 276 | Ile 276 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 589 |
| Total formula weight | 27773299.4 | |
| Non-Polymers* | Number of molecules | 510 |
| Total formula weight | 168368.6 | |
| All* | Total formula weight | 27941668.0 |
