9UMG
Cryo-EM structure of VTC complex(Vtc5/Vtc4/Vtc3/Vtc1)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Vacuolar transporter chaperone complex subunit 1 | polymer | 118 | 13253.8 | 1 | UniProt (P40046) Pfam (PF02656) | Saccharomyces cerevisiae S288C | Negative regulator of CDC42 protein 1,Phosphate metabolism protein 4,SPX-dependent polyphosphate polymerase VTC subunit 1,Vacuolar membrane polyphosphate polymerase accessory subunit 1,PolyP polymerase |
| 2 | B, C (B, C) | Vacuolar transporter chaperone complex subunit 1 | polymer | 103 | 11592.7 | 2 | UniProt (P40046) Pfam (PF02656) | Saccharomyces cerevisiae S288C | Negative regulator of CDC42 protein 1,Phosphate metabolism protein 4,SPX-dependent polyphosphate polymerase VTC subunit 1,Vacuolar membrane polyphosphate polymerase accessory subunit 1,PolyP polymerase |
| 3 | D (D) | Vacuolar transporter chaperone complex subunit 4 | polymer | 527 | 60283.6 | 1 | UniProt (P47075) Pfam (PF09359) Pfam (PF02656) | Saccharomyces cerevisiae S288C | Phosphate metabolism protein 3,Polyphosphate kinase,SPX-dependent polyphosphate polymerase VTC subunit 4,Vacuolar membrane polyphosphate polymerase catalytic subunit,PolyP polymerase |
| 4 | E (E) | Vacuolar transporter chaperone 3 complex subunit 3 | polymer | 604 | 69612.7 | 1 | UniProt (Q02725) Pfam (PF09359) Pfam (PF02656) | Saccharomyces cerevisiae S288C | Phosphate metabolism protein 2,SPX-dependent polyphosphate polymerase VTC subunit 3,Vacuolar membrane polyphosphate polymerase accessory subunit 3,PolyP polymerase |
| 5 | F (F) | Vacuole transporter chaperone complex subunit 5 | polymer | 110 | 12434.9 | 1 | UniProt (P38966) | Saccharomyces cerevisiae S288C | Vacuolar membrane polyphosphate polymerase regulatory subunit 5,PolyP polymerase |
| 6 | G, H, I (D, E) | INOSITOL HEXAKISPHOSPHATE | non-polymer | 660.0 | 3 | Chemie (IHP) |
Sequence modifications
D: 196 - 721 (UniProt: P47075)
| PDB | External Database | Details |
|---|---|---|
| Ala 264 | Arg 264 | engineered mutation |
| Ala 266 | Arg 266 | engineered mutation |
| Asn 426 | Glu 426 | engineered mutation |
| Leu 722 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 178770.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1980.1 | |
| All* | Total formula weight | 180750.6 |
