9UHX
Cryo-EM structure of the human adenosine A2A receptor bound to the antagonist ZM-241385 resolved via the fusion/crosslinking strategy
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Adenosine receptor A2a | polymer | 331 | 36733.5 | 1 | UniProt (P29274) Pfam (PF00001) | Homo sapiens (human) | |
| 2 | B (B) | Green fluorescent protein | polymer | 196 | 22388.3 | 1 | UniProt (A0A059PIQ0) | Aequorea victoria | |
| 3 | C (C) | GFP-clamp | polymer | 294 | 31064.6 | 1 | synthetic construct | ||
| 4 | D (A) | 4-{2-[(7-amino-2-furan-2-yl[1,2,4]triazolo[1,5-a][1,3,5]triazin-5-yl)amino]ethyl}phenol | non-polymer | 337.3 | 1 | Chemie (ZMA) PubChem (176407) |
Sequence modifications
A: 2 - 208 (UniProt: P29274)
A: 225 - 317 (UniProt: P29274)
B: 4 - 198 (UniProt: A0A059PIQ0)
A: 225 - 317 (UniProt: P29274)
| PDB | External Database | Details |
|---|---|---|
| Gly 1001 | - | linker |
| His 1002 | - | linker |
| Tyr 1003 | - | linker |
| Leu 1004 | - | linker |
| Ser 1005 | - | linker |
| Thr 1006 | - | linker |
| Gln 1007 | - | linker |
| Thr 1008 | - | linker |
| Ser 1009 | - | linker |
| Leu 1010 | - | linker |
| Ser 1011 | - | linker |
| Lys 1012 | - | linker |
| Asp 1013 | - | linker |
| Leu 1014 | - | linker |
| Asn 1015 | - | linker |
| Glu 1016 | - | linker |
| Lys 1017 | - | linker |
| Arg 1018 | - | linker |
| Asp 1019 | - | linker |
| His 1020 | - | linker |
| Met 1021 | - | linker |
| Val 1022 | - | linker |
| Leu 1023 | - | linker |
| Leu 1024 | - | linker |
| Glu 1025 | - | linker |
| Phe 1026 | - | linker |
| Val 1027 | - | linker |
| Thr 1028 | - | linker |
| Ala 1029 | - | linker |
| Ala 1030 | - | linker |
| Gly 1031 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Gly 2 | - | expression tag |
| Thr 3 | - | expression tag |
| Ile 18 | Val 16 | conflict |
| Arg 32 | Ser 30 | conflict |
| Tyr 41 | Asn 39 | conflict |
| Cro 68 | Thr 65 | conflict |
| - | Tyr 66 | deletion |
| - | Gly 67 | deletion |
| Ser 74 | Ala 72 | conflict |
| Arg 82 | Gln 80 | conflict |
| Tyr 101 | Ser 99 | conflict |
| Met 155 | Thr 153 | conflict |
| Asn 160 | Lys 158 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 90186.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 337.3 | |
| All* | Total formula weight | 90523.8 |
