9U98

Glycoside hydrolase family 1 beta-glucosidase (E318G mutant) from Streptomyces griseus (sophorose complex)

Replaces:  8KAQ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Putative beta-glucosidase	polymer	414	45690.6	4	UniProt (B1W1N0) Pfam (PF00232)	Streptomyces griseus subsp. griseus NBRC 13350
2	E, H (E, H)	beta-D-glucopyranose-(1-2)-beta-D-glucopyranose	branched		342.3	2	In PDB GlyTouCan (G26080VZ)
3	F, G, I (F, G, I)	beta-D-glucopyranose-(1-2)-alpha-D-glucopyranose	branched		342.3	3	In PDB GlyTouCan (G71571NA)
4	J, M, P (A, B, C)	TRIETHYLENE GLYCOL	non-polymer		150.2	3	Chemie (PGE)
5	K, L, N, O, Q (A, B, C)	CHLORIDE ION	non-polymer		35.5	5	Chemie (CL)
6	R (D)	beta-D-glucopyranose	non-polymer		180.2	1	Chemie (BGC)
7	S, T, U, V (A, B, C, D)	water	water		18.0	530	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 406 (UniProt: B1W1N0)

PDB	External Database	Details
Gly 318	Glu 318	engineered mutation
Leu 407	-	expression tag
Glu 408	-	expression tag
His 409	-	expression tag
His 410	-	expression tag
His 411	-	expression tag
His 412	-	expression tag
His 413	-	expression tag
His 414	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	182762.4
Branched	Number of molecules	5
	Total formula weight	1711.5
Non-Polymers*	Number of molecules	9
	Total formula weight	807.9
All*	Total formula weight	185281.8

*Water molecules are not included.