9U5U

The 1:1 cryo-EM structure of BAP1/ASXL1-K351Ub in complex with H2AK119Ub nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15273.8	2	UniProt (Q71DI3)	Homo sapiens (human)	H3-clustered histone 13,H3-clustered histone 14,H3-clustered histone 15,Histone H3/m,Histone H3/o
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62805)	Homo sapiens (human)
3	C, G (C, G)	Histone H2A type 1-B/E	polymer	129	14034.4	2	UniProt (P04908)	Homo sapiens (human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H (D, H)	Histone H2B type 1-K	polymer	125	13790.0	2	UniProt (O60814)	Homo sapiens (human)	H2B K,HIRA-interacting protein 1
5	I (I)	DNA (147-MER)	polymer	147	45604.0	1		Homo sapiens
6	J (J)	DNA (147-MER)	polymer	147	45145.8	1		Homo sapiens
7	K (L)	Ubiquitin carboxyl-terminal hydrolase BAP1	polymer	711	78205.9	1	UniProt (Q92560)	Homo sapiens (human)	BRCA1-associated protein 1,Cerebral protein 6
8	L, N (M, O)	Ubiquitin	polymer	76	8576.8	2	UniProt (P62979)	Homo sapiens (human)	Donor Ub
9	M (N)	Polycomb group protein ASXL1	polymer	159	18507.7	1	UniProt (Q8IXJ9)	Homo sapiens (human)	Additional sex combs-like protein 1

Sequence modifications

A, E: 1 - 135 (UniProt: Q71DI3)

PDB	External Database	Details
Ser 110	Cys 111	conflict

N: 238 - 390 (UniProt: Q8IXJ9)

PDB	External Database	Details
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	313339.9
All*	Total formula weight	313339.9

*Water molecules are not included.