9U3P

Cryo-EM structure of human AC9-Gs complex (soluble domain)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Adenylate cyclase type 9,Protein M2-1	polymer	1622	181560.2	1	UniProt (O60503) UniProt (A0A1S5SHT2) Pfam (PF00211)	Homo sapiens (human)	ATP pyrophosphate-lyase 9,Adenylate cyclase type IX,ACIX,Adenylyl cyclase 9,AC9,Envelope-associated 22 kDa protein,Transcription antitermination factor M2-1
2	B (B)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	423	49331.2	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
3	C (B)	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE	non-polymer		539.2	1	Chemie (GSP)
4	D (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 1 - 1353 (UniProt: O60503)

PDB	External Database	Details
Gly 1354	-	linker
Ser 1355	-	linker
Asn 1356	-	linker
Ser 1357	-	linker
Glu 1358	-	linker
Asn 1359	-	linker
Leu 1360	-	linker
Tyr 1361	-	linker
Phe 1362	-	linker
Gln 1363	-	linker
Gly 1364	-	linker
Ser 1365	-	linker

A: 1366 - 1604 (UniProt: A0A1S5SHT2)

PDB	External Database	Details
His 1605	-	expression tag
His 1606	-	expression tag
His 1607	-	expression tag
His 1608	-	expression tag
His 1609	-	expression tag
His 1610	-	expression tag
His 1611	-	expression tag
His 1612	-	expression tag
His 1613	-	expression tag
His 1614	-	expression tag
Asp 1615	-	expression tag
Tyr 1616	-	expression tag
Lys 1617	-	expression tag
Asp 1618	-	expression tag
Asp 1619	-	expression tag
Asp 1620	-	expression tag
Asp 1621	-	expression tag
Lys 1622	-	expression tag

B: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Ser 395	-	expression tag
Asn 396	-	expression tag
Ser 397	-	expression tag
Glu 398	-	expression tag
Asn 399	-	expression tag
Leu 400	-	expression tag
Tyr 401	-	expression tag
Phe 402	-	expression tag
Gln 403	-	expression tag
Gly 404	-	expression tag
Ser 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag
His 408	-	expression tag
His 409	-	expression tag
His 410	-	expression tag
His 411	-	expression tag
His 412	-	expression tag
His 413	-	expression tag
His 414	-	expression tag
His 415	-	expression tag
Asp 416	-	expression tag
Tyr 417	-	expression tag
Lys 418	-	expression tag
Asp 419	-	expression tag
Asp 420	-	expression tag
Asp 421	-	expression tag
Asp 422	-	expression tag
Lys 423	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	230891.4
Non-Polymers*	Number of molecules	2
	Total formula weight	563.6
All*	Total formula weight	231454.9

*Water molecules are not included.