9TY2

Crystal structure of a Picomolar Nanobody in complex with Maltose Binding Protein, MBP, and maltose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Maltose/maltodextrin-binding periplasmic protein	polymer	387	42554.8	1	UniProt (P0AEY0) Pfam (PF01547)	Escherichia coli	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
2	B (B)	Nanobody, single fragment heavy chain antibody, NbCM1, sdAbCM1	polymer	128	13997.7	1		Vicugna pacos
3	C (C)	alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900018) GlyTouCan (G66120GK)
4	D, E (A, B)	water	water		18.0	372	Chemie (HOH)

Sequence modifications

A: 2 - 367 (UniProt: P0AEY0)

PDB	External Database	Details
Met 1	-	initiating methionine
Val 313	Ala 338	conflict
Asn 368	-	expression tag
Ser 369	-	expression tag
Ser 370	-	expression tag
Ser 371	-	expression tag
Asn 372	-	expression tag
Asn 373	-	expression tag
Asn 374	-	expression tag
Asn 375	-	expression tag
Asn 376	-	expression tag
Asn 377	-	expression tag
Asn 378	-	expression tag
Asn 379	-	expression tag
Asn 380	-	expression tag
Asn 381	-	expression tag
Leu 382	-	expression tag
Gly 383	-	expression tag
Ile 384	-	expression tag
Glu 385	-	expression tag
Gly 386	-	expression tag
Arg 387	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56552.5
Branched	Number of molecules	1
	Total formula weight	342.3
All*	Total formula weight	56894.8

*Water molecules are not included.