9TY2
Crystal structure of a Picomolar Nanobody in complex with Maltose Binding Protein, MBP, and maltose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-07 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.602, 52.158, 77.144 |
| Unit cell angles | 90.00, 92.08, 90.00 |
Refinement procedure
| Resolution | 52.177 - 1.750 |
| Rwork | 0.177 |
| R-free | 0.23620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.230 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.164 | 1.160 |
| Number of reflections | 54091 | 2962 |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 98.0 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.990 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M MIB (Sodium malonate dibasic monohydrate, Imidazole, Boric acid) pH 7.0, 25 % w/v PEG 1500 |
