9TWV

Complex of E. coli leucyl-tRNA synthetase AN3365-resistant mutant D345A bound to tRNA(leu)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Leucine--tRNA ligase	polymer	880	99472.0	1	UniProt (P07813) Pfam (PF09334) Pfam (PF13603) Pfam (PF00133) Pfam (PF08264)	Escherichia coli	Leucyl-tRNA synthetase,LeuRS
2	B (B)	tRNA(leu)	polymer	87	28052.7	1	GenBank (1231762938)	Escherichia coli
3	C (A)	(2~{S})-2-azanyl-~{N}-[azanyl-(3-fluorophenyl)-oxidanylidene-$l^{6}-sulfanylidene]-4-methyl-pentanamide	non-polymer		287.4	1	Chemie (A1JX6)
4	D (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG) PubChem (8117)
5	E (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)
6	F, G (A, B)	water	water		18.0	465	Chemie (HOH)

Sequence modifications

A: 1 - 860 (UniProt: P07813)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 345	Asp 345	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	127524.7
Non-Polymers*	Number of molecules	3
	Total formula weight	417.8
All*	Total formula weight	127942.4

*Water molecules are not included.