9TKO

30S pre-initiation complex with IF2 bound (30S PIC-B)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (AA)	Small ribosomal subunit protein uS2	polymer	241	26781.7	1	UniProt (P0A7V0)	Escherichia coli	30S ribosomal protein S2
2	B (AB)	Small ribosomal subunit protein uS3	polymer	233	26031.3	1	UniProt (P0A7V3)	Escherichia coli	30S ribosomal protein S3
3	C (AC)	Small ribosomal subunit protein uS4	polymer	206	23514.2	1	UniProt (P0A7V8)	Escherichia coli	30S ribosomal protein S4
4	D (AD)	Small ribosomal subunit protein uS5	polymer	167	17629.4	1	UniProt (P0A7W1)	Escherichia coli	30S ribosomal protein S5
5	E (AE)	Small ribosomal subunit protein bS6, fully modified isoform	polymer	135	15727.5	1	UniProt (P02358)	Escherichia coli
6	F (AF)	Small ribosomal subunit protein uS7	polymer	179	20055.2	1	UniProt (P02359)	Escherichia coli	30S ribosomal protein S7
7	G (AG)	Small ribosomal subunit protein uS8	polymer	130	14146.6	1	UniProt (P0A7W7)	Escherichia coli	30S ribosomal protein S8
8	H (AH)	Small ribosomal subunit protein uS9	polymer	130	14886.3	1	UniProt (P0A7X3)	Escherichia coli	30S ribosomal protein S9
9	I (AI)	Small ribosomal subunit protein uS10	polymer	103	11755.6	1	UniProt (P0A7R5)	Escherichia coli	30S ribosomal protein S10,Transcription termination/antitermination protein NusE
10	J (AJ)	Small ribosomal subunit protein uS11	polymer	129	13871.0	1	UniProt (P0A7R9)	Escherichia coli	30S ribosomal protein S11
11	K (AK)	Small ribosomal subunit protein uS12	polymer	124	13768.2	1	UniProt (P0A7S3)	Escherichia coli	30S ribosomal protein S12
12	L (AL)	Small ribosomal subunit protein uS13	polymer	118	13128.5	1	UniProt (P0A7S9)	Escherichia coli	30S ribosomal protein S13
13	M (AM)	Small ribosomal subunit protein uS14	polymer	101	11606.6	1	UniProt (P0AG59)	Escherichia coli	30S ribosomal protein S14
14	N (AN)	Small ribosomal subunit protein uS15	polymer	89	10290.8	1	UniProt (P0ADZ4)	Escherichia coli	30S ribosomal protein S15
15	O (AO)	Small ribosomal subunit protein bS16	polymer	82	9207.6	1	UniProt (P0A7T3)	Escherichia coli	30S ribosomal protein S16
16	P (AP)	Small ribosomal subunit protein uS17	polymer	83	9593.3	1	UniProt (P0AG63)	Escherichia coli	30S ribosomal protein S17
17	Q (AQ)	Small ribosomal subunit protein bS18	polymer	75	9005.5	1	UniProt (P0A7T7)	Escherichia coli	30S ribosomal protein S18
18	R (AR)	Small ribosomal subunit protein uS19	polymer	92	10455.4	1	UniProt (P0A7U3)	Escherichia coli	30S ribosomal protein S19
19	S (AT)	Small ribosomal subunit protein bS20	polymer	87	9708.5	1	UniProt (P0A7U7)	Escherichia coli	30S ribosomal protein S20
20	T (AU)	Small ribosomal subunit protein bS21	polymer	71	8524.0	1	UniProt (P68679)	Escherichia coli	30S ribosomal protein S21
21	U (D1)	16S Ribosomal RNA	polymer	1540	499031.6	1	GenBank (CP194060.1)	Escherichia coli
22	V (D4)	mRNA	polymer	56	18057.8	1		Escherichia coli
23	W (D5)	tRNAfMet	polymer	77	24802.8	1	GenBank (1848851804)	Escherichia coli
24	X (F2)	Translation initiation factor IF-2	polymer	896	98327.1	1	UniProt (P0A705)	Escherichia coli
25	Y (F2)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
26	Z (F2)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)

Sequence modifications

F2: 2 - 890 (UniProt: P0A705)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	929906.1
Non-Polymers*	Number of molecules	2
	Total formula weight	547.5
All*	Total formula weight	930453.6

*Water molecules are not included.