9TKO

30S pre-initiation complex with IF2 bound (30S PIC-B)

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Detector	GATAN K3 (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3
Number of Particle	1889196
Software	cisTEM,FREALIGN,PHENIX,CTFFIND
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION