9TI4
High resolution Cryo-EM structure of human complex I in mitochondria
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial | polymer | 434 | 47680.3 | 1 | UniProt (P49821) Pfam (PF01512) Pfam (PF22461) Pfam (PF10589) | Homo sapiens (human) | NDUFV1,Complex I-51kD,CI-51kD,NADH dehydrogenase flavoprotein 1,NADH-ubiquinone oxidoreductase 51 kDa subunit |
| 2 | B (D) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial | polymer | 176 | 20314.0 | 1 | UniProt (O00217) Pfam (PF12838) | Homo sapiens (human) | Complex I-23kD,CI-23kD,NADH-ubiquinone oxidoreductase 23 kDa subunit,TYKY subunit |
| 3 | C (E) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial | polymer | 161 | 18449.5 | 1 | UniProt (O75251) Pfam (PF01058) | Homo sapiens (human) | Complex I-20kD,CI-20kD,NADH-ubiquinone oxidoreductase 20 kDa subunit,PSST subunit |
| 4 | D (F) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 | polymer | 118 | 14205.5 | 1 | UniProt (P56556) Pfam (PF13233) | Homo sapiens (human) | Complex I-B14,CI-B14,LYR motif-containing protein 6,NADH-ubiquinone oxidoreductase B14 subunit |
| 5 | E (G) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 | polymer | 85 | 9722.1 | 1 | UniProt (O43678) Pfam (PF05047) | Homo sapiens (human) | Complex I-B8,CI-B8,NADH-ubiquinone oxidoreductase B8 subunit |
| 6 | F, U (H, X) | Acyl carrier protein, mitochondrial | polymer | 86 | 9976.4 | 2 | UniProt (O14561) Pfam (PF00550) | Homo sapiens (human) | ACP,CI-SDAP,NADH-ubiquinone oxidoreductase 9.6 kDa subunit |
| 7 | G (I) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 | polymer | 112 | 13119.2 | 1 | UniProt (Q16718) Pfam (PF04716) | Homo sapiens (human) | Complex I subunit B13,Complex I-13kD-B,CI-13kD-B,NADH-ubiquinone oxidoreductase 13 kDa-B subunit |
| 8 | H (J) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial | polymer | 341 | 38848.1 | 1 | UniProt (Q16795) Pfam (PF01370) | Homo sapiens (human) | Complex I-39kD,CI-39kD,NADH-ubiquinone oxidoreductase 39 kDa subunit |
| 9 | I (K) | NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial | polymer | 39 | 4623.1 | 1 | UniProt (P56181) Pfam (PF15880) | Homo sapiens (human) | Complex I-9kD,CI-9kD,NADH-ubiquinone oxidoreductase 9 kDa subunit,Renal carcinoma antigen NY-REN-4 |
| 10 | J (L) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial | polymer | 121 | 14064.9 | 1 | UniProt (O43181) Pfam (PF04800) | Homo sapiens (human) | Complex I-18 kDa,CI-18 kDa,Complex I-AQDQ,CI-AQDQ,NADH-ubiquinone oxidoreductase 18 kDa subunit |
| 11 | K (M) | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial | polymer | 687 | 75471.5 | 1 | UniProt (P28331) Pfam (PF13510) Pfam (PF10588) Pfam (PF22117) Pfam (PF22151) Pfam (PF00384) Pfam (PF09326) | Homo sapiens (human) | Complex I-75kD,CI-75kD |
| 12 | L (N) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 | polymer | 144 | 17011.3 | 1 | UniProt (Q9UI09) Pfam (PF05071) | Homo sapiens (human) | 13 kDa differentiation-associated protein,Complex I-B17.2,CI-B17.2,CIB17.2,NADH-ubiquinone oxidoreductase subunit B17.2 |
| 13 | M (O) | NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial | polymer | 212 | 23430.9 | 1 | UniProt (P19404) Pfam (PF01257) | Homo sapiens (human) | NDUFV2,NADH-ubiquinone oxidoreductase 24 kDa subunit |
| 14 | N (P) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial | polymer | 208 | 24432.7 | 1 | UniProt (O75489) Pfam (PF00329) | Homo sapiens (human) | Complex I-30kD,CI-30kD,NADH-ubiquinone oxidoreductase 30 kDa subunit |
| 15 | O (Q) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial | polymer | 430 | 49249.5 | 1 | UniProt (O75306) Pfam (PF00346) | Homo sapiens (human) | Complex I-49kD,CI-49kD,NADH-ubiquinone oxidoreductase 49 kDa subunit |
| 16 | P (S) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 | polymer | 70 | 8084.4 | 1 | UniProt (O15239) Pfam (PF15879) | Homo sapiens (human) | Complex I-MWFE,CI-MWFE,NADH-ubiquinone oxidoreductase MWFE subunit |
| 17 | Q (T) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial | polymer | 96 | 10717.0 | 1 | UniProt (O75380) Pfam (PF10276) | Homo sapiens (human) | Complex I-13kD-A,CI-13kD-A,NADH-ubiquinone oxidoreductase 13 kDa-A subunit |
| 18 | R (U) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 | polymer | 83 | 9156.6 | 1 | UniProt (O95167) Pfam (PF14987) | Homo sapiens (human) | Complex I-B9,CI-B9,NADH-ubiquinone oxidoreductase B9 subunit |
| 19 | S (V) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 | polymer | 140 | 14736.9 | 1 | UniProt (Q86Y39) | Homo sapiens (human) | Complex I-B14.7,CI-B14.7,NADH-ubiquinone oxidoreductase subunit B14.7 |
| 20 | T (W) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 | polymer | 140 | 16360.9 | 1 | UniProt (Q9P0J0) Pfam (PF06212) | Homo sapiens (human) | Cell death regulatory protein GRIM-19,Complex I-B16.6,CI-B16.6,Gene associated with retinoic and interferon-induced mortality 19 protein,GRIM-19,Gene associated with retinoic and IFN-induced mortality 19 protein,NADH-ubiquinone oxidoreductase B16.6 subunit |
| 21 | V (Y) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial | polymer | 61 | 7624.5 | 1 | UniProt (O95178) Pfam (PF14813) | Homo sapiens (human) | Complex I-AGGG,CI-AGGG,NADH-ubiquinone oxidoreductase AGGG subunit |
| 22 | W (Z) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 | polymer | 83 | 9575.9 | 1 | UniProt (O43676) Pfam (PF08122) | Homo sapiens (human) | Complex I-B12,CI-B12,NADH-ubiquinone oxidoreductase B12 subunit |
| 23 | X (a) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial | polymer | 138 | 16573.2 | 1 | UniProt (O43674) Pfam (PF09781) | Homo sapiens (human) | Complex I-SGDH,CI-SGDH,NADH-ubiquinone oxidoreductase SGDH subunit |
| 24 | Y (b) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 | polymer | 120 | 14491.0 | 1 | UniProt (O95139) Pfam (PF09782) | Homo sapiens (human) | Complex I-B17,CI-B17,NADH-ubiquinone oxidoreductase B17 subunit |
| 25 | Z (c) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial | polymer | 152 | 18094.4 | 1 | UniProt (O95169) Pfam (PF05821) | Homo sapiens (human) | Complex I-ASHI,CI-ASHI,NADH-ubiquinone oxidoreductase ASHI subunit |
| 26 | AA (d) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 | polymer | 169 | 20489.3 | 1 | UniProt (O96000) Pfam (PF10249) | Homo sapiens (human) | Complex I-PDSW,CI-PDSW,NADH-ubiquinone oxidoreductase PDSW subunit |
| 27 | BA (e) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial | polymer | 107 | 12590.1 | 1 | UniProt (Q9NX14) Pfam (PF10183) | Homo sapiens (human) | Complex I-ESSS,CI-ESSS,NADH-ubiquinone oxidoreductase ESSS subunit,Neuronal protein 17.3,Np17.3,p17.3 |
| 28 | CA (f) | NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial | polymer | 49 | 5904.8 | 1 | UniProt (O43677) Pfam (PF15088) | Homo sapiens (human) | Complex I-KFYI,CI-KFYI,NADH-ubiquinone oxidoreductase KFYI subunit |
| 29 | DA (g) | NADH dehydrogenase [ubiquinone] 1 subunit C2 | polymer | 119 | 14209.5 | 1 | UniProt (O95298) Pfam (PF06374) | Homo sapiens (human) | Complex I-B14.5b,CI-B14.5b,Human lung cancer oncogene 1 protein,HLC-1,NADH-ubiquinone oxidoreductase subunit B14.5b |
| 30 | EA (h) | NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 | polymer | 105 | 12445.4 | 1 | UniProt (O43920) Pfam (PF10200) | Homo sapiens (human) | Complex I-15 kDa,CI-15 kDa,NADH-ubiquinone oxidoreductase 15 kDa subunit |
| 31 | FA (i) | NADH-ubiquinone oxidoreductase chain 2 | polymer | 347 | 38980.6 | 1 | UniProt (P03891) Pfam (PF00361) Pfam (PF06444) | Homo sapiens (human) | NADH dehydrogenase subunit 2 |
| 32 | GA (j) | NADH-ubiquinone oxidoreductase chain 3 | polymer | 115 | 13190.0 | 1 | UniProt (P03897) Pfam (PF00507) | Homo sapiens (human) | NADH dehydrogenase subunit 3 |
| 33 | HA (k) | NADH-ubiquinone oxidoreductase chain 4L | polymer | 98 | 10745.1 | 1 | UniProt (P03901) Pfam (PF00420) | Homo sapiens (human) | NADH dehydrogenase subunit 4L,ND4L |
| 34 | IA (l) | NADH-ubiquinone oxidoreductase chain 5 | polymer | 601 | 66836.6 | 1 | UniProt (P03915) Pfam (PF00662) Pfam (PF00361) Pfam (PF06455) | Homo sapiens (human) | NADH dehydrogenase subunit 5 |
| 35 | JA (m) | NADH-ubiquinone oxidoreductase chain 6 | polymer | 173 | 18512.9 | 1 | UniProt (P03923) Pfam (PF00499) | Homo sapiens (human) | NADH dehydrogenase subunit 6 |
| 36 | KA (n) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 | polymer | 54 | 6514.6 | 1 | UniProt (O75438) Pfam (PF08040) | Homo sapiens (human) | Complex I-MNLL,CI-MNLL,NADH-ubiquinone oxidoreductase MNLL subunit |
| 37 | LA (o) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 | polymer | 128 | 15100.4 | 1 | UniProt (O95168) Pfam (PF07225) | Homo sapiens (human) | Complex I-B15,CI-B15,NADH-ubiquinone oxidoreductase B15 subunit |
| 38 | MA (p) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 | polymer | 173 | 21246.2 | 1 | UniProt (Q9Y6M9) Pfam (PF05347) | Homo sapiens (human) | Complex I-B22,CI-B22,LYR motif-containing protein 3,NADH-ubiquinone oxidoreductase B22 subunit |
| 39 | NA (r) | NADH-ubiquinone oxidoreductase chain 4 | polymer | 459 | 51613.5 | 1 | UniProt (P03905) Pfam (PF01059) Pfam (PF00361) | Homo sapiens (human) | NADH dehydrogenase subunit 4 |
| 40 | OA (s) | NADH-ubiquinone oxidoreductase chain 1 | polymer | 316 | 35448.0 | 1 | UniProt (P03886) Pfam (PF00146) | Homo sapiens (human) | NADH dehydrogenase subunit 1 |
| 41 | PA (u) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 | polymer | 171 | 20007.9 | 1 | UniProt (P51970) Pfam (PF06747) | Homo sapiens (human) | Complex I-19kD,CI-19kD,Complex I-PGIV,CI-PGIV,NADH-ubiquinone oxidoreductase 19 kDa subunit |
| 42 | QA (v) | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 | polymer | 121 | 14854.2 | 1 | UniProt (P17568) Pfam (PF05676) | Homo sapiens (human) | Cell adhesion protein SQM1,Complex I-B18,CI-B18,NADH-ubiquinone oxidoreductase B18 subunit |
| 43 | RA (w) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial | polymer | 320 | 37200.3 | 1 | UniProt (O95299) Pfam (PF01712) | Homo sapiens (human) | Complex I-42kD,CI-42kD,NADH-ubiquinone oxidoreductase 42 kDa subunit |
| 44 | SA (t) | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 | polymer | 113 | 12571.4 | 1 | UniProt (O95182) Pfam (PF07347) | Homo sapiens (human) | Complex I-B14.5a,CI-B14.5a,NADH-ubiquinone oxidoreductase subunit B14.5a |
| 45 | CB, DB, TA, VA, WA... (M, C, D, E) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 6 | Chemie (SF4) | |||
| 46 | UA (C) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 1 | Chemie (FMN) PubChem (710) PubChem (23585816) PubChem (8965) PubChem (51435500) PubChem (51435503) PubChem (643976) PubChem (5702760) PubChem (7048779) PubChem (7048781) PubChem (15559251) | |||
| 47 | BB, IB, LB, QB, RB... (J, V, W, i, l...) | 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine | non-polymer | 744.0 | 7 | Chemie (PEE) PubChem (108229) PubChem (44891344) PubChem (54095275) PubChem (14238554) PubChem (5909904) PubChem (9546757) PubChem (6437392) PubChem (99655478) PubChem (99655479) PubChem (9546744) PubChem (138113035) PubChem (171729549) | |||
| 48 | MB, ZA (X, F) | S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate | non-polymer | 540.7 | 2 | Chemie (8Q1) PubChem (119058169) PubChem (122362359) PubChem (162826280) | |||
| 49 | AB (J) | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 1 | Chemie (NDP) | |||
| 50 | EB, GB (M, O) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 51 | FB, NB, OB, SB, WB... (N, g, j, o, r...) | (9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL | non-polymer | 767.1 | 7 | Chemie (PLX) PubChem (49867511) | |||
| 52 | HB (T) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 53 | JB, KB, PB, UB, VB... (V, i, l, u) | CARDIOLIPIN | non-polymer | 1464.0 | 6 | Chemie (CDL) PubChem (17753886) PubChem (5287898) | |||
| 54 | AC (w) | 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (DGT) PubChem (177678918) PubChem (136110594) PubChem (135398599) PubChem (135402022) PubChem (135566345) PubChem (136254580) PubChem (136633653) PubChem (136828797) PubChem (136828798) PubChem (137226754) PubChem (137257907) PubChem (171699498) | |||
| 55 | BC (w) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 56 | CC (X) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
H, X: 72 - 156 (UniProt: O14561)
b: 2 - 122 (UniProt: O95139)
| PDB | External Database | Details |
|---|---|---|
| Met 71 | - | initiating methionine |
| PDB | External Database | Details |
|---|---|---|
| - | Met 40 | deletion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 45 |
| Total formula weight | 944451.2 | |
| Non-Polymers* | Number of molecules | 35 |
| Total formula weight | 24703.9 | |
| All* | Total formula weight | 969155.1 |
