9TH6

nsp14 of SARS-CoV-2 in complex with a camelid nanobody

Replaces:  9H01

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine-N7 methyltransferase nsp14	polymer	547	62264.0	1	UniProt (P0DTD1) Pfam (PF06471)	Severe acute respiratory syndrome coronavirus 2	Non-structural protein 14,nsp14,Proofreading exoribonuclease nsp14,ExoN
2	B (B)	vhh anti-nsp14 of SARS-CoV-2	polymer	132	14290.9	1		Lama glama
3	C, D, E (A)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
4	F, G (A, B)	water	water		18.0	120	Chemie (HOH)

Sequence modifications

A: 1 - 527 (UniProt: P0DTD1)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76554.9
Non-Polymers*	Number of molecules	3
	Total formula weight	196.2
All*	Total formula weight	76751.1

*Water molecules are not included.