Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.514, 80.801, 138.117 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.740 - 2.267 |
| R-factor | 0.2361 |
| Rwork | 0.233 |
| R-free | 0.28970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | autoPROC (1.0.5) |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.743 | 2.537 |
| High resolution limit [Å] | 2.267 | 2.267 |
| Rmerge | 0.134 | 1.735 |
| Rmeas | 0.140 | 1.822 |
| Rpim | 0.041 | 0.550 |
| Number of reflections | 209521 | 9522 |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 93.2 | |
| Redundancy | 12 | |
| CC(1/2) | 0.999 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 22,5-26,25% w/v PEG 3350, 0.1 M Bis Tris propane, 0.2 M sodium fluoride |
