9TAN
Local refinement of E. coli Complex I D79N NuoA mutant hydrophilic domain in LMNG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | NADH-quinone oxidoreductase subunit B | polymer | 220 | 25081.8 | 1 | UniProt (P0AFC7) Pfam (PF01058) | Escherichia coli BW25113 | NADH dehydrogenase I subunit B,NDH-1 subunit B,NUO2 |
| 2 | B (C) | NADH-quinone oxidoreductase subunit C/D | polymer | 600 | 68780.5 | 1 | UniProt (P33599) Pfam (PF00329) Pfam (PF00346) | Escherichia coli BW25113 | NADH dehydrogenase I subunit C/D,NDH-1 subunit C/D,NUO3/NUO4 |
| 3 | C (E) | NADH-quinone oxidoreductase subunit E | polymer | 166 | 18614.0 | 1 | UniProt (P0AFD1) Pfam (PF01257) | Escherichia coli BW25113 | NADH dehydrogenase I subunit E,NDH-1 subunit E,NUO5 |
| 4 | D (F) | NADH-quinone oxidoreductase subunit F | polymer | 461 | 51208.4 | 1 | UniProt (P31979) Pfam (PF01512) Pfam (PF10589) | Escherichia coli BW25113 | NADH dehydrogenase I subunit F,NDH-1 subunit F,NUO6 |
| 5 | E (G) | NADH-quinone oxidoreductase subunit G | polymer | 910 | 100663.6 | 1 | UniProt (P33602) Pfam (PF04879) Pfam (PF00384) Pfam (PF01568) Pfam (PF13510) Pfam (PF10588) Pfam (PF22117) | Escherichia coli BW25113 | NADH dehydrogenase I subunit G,NDH-1 subunit G,NUO7 |
| 6 | F (I) | NADH-quinone oxidoreductase subunit I | polymer | 180 | 20562.8 | 1 | UniProt (P0AFD6) Pfam (PF00037) | Escherichia coli BW25113 | NADH dehydrogenase I subunit I,NDH-1 subunit I,NUO9 |
| 7 | G, J, L, M, N... (B, F, G, I) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 7 | Chemie (SF4) | |||
| 8 | H, O (E, G) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 9 | I, P, Q, T (E, G, I) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) PubChem (271) | |||
| 10 | K (F) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 1 | Chemie (FMN) PubChem (643976) | |||
| 11 | U, V, W, X, Y... (B, C, E, F, G...) | water | water | 18.0 | 296 | Chemie (HOH) |
Sequence modifications
C: 5 - 600 (UniProt: P33599)
F: 2 - 445 (UniProt: P31979)
G: 3 - 910 (UniProt: P33602)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Val 2 | - | expression tag |
| Asn 3 | - | expression tag |
| Asn 4 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| Arg -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Ser -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| Thr -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Arg 0 | - | expression tag |
| Ala 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Leu 2 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 284911.0 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 3429.8 | |
| All* | Total formula weight | 288340.8 |
