9T6L

Cryo-EM structure of the human LENG8-PCID2-DSS1 complex bound to UAP56 and RRP1B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Spliceosome RNA helicase DDX39B	polymer	432	49412.6	1	UniProt (Q13838) Pfam (PF00270) Pfam (PF00271)	Homo sapiens (human)	56 kDa U2AF65-associated protein,ATP-dependent RNA helicase p47,DEAD box protein UAP56,HLA-B-associated transcript 1 protein
2	B (C)	Leukocyte receptor cluster member 8,Ribosomal RNA processing protein 1 homolog B	polymer	273	30940.7	1	UniProt (Q96PV6) UniProt (Q14684) Pfam (PF03399)	Homo sapiens (human)	RRP1-like protein B
3	C (D)	PCI domain-containing protein 2	polymer	402	46371.2	1	UniProt (Q5JVF3) Pfam (PF01399)	Homo sapiens (human)	CSN12-like protein
4	D (E)	26S proteasome complex subunit SEM1	polymer	70	8284.6	1	UniProt (P60896) Pfam (PF05160)	Homo sapiens (human)	26S proteasome complex subunit DSS1,Deleted in split hand/split foot protein 1,Split hand/foot deleted protein 1,Split hand/foot malformation type 1 protein

Sequence modifications

B: 1 - 428 (UniProt: Q13838)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag

C: 550 - 800 (UniProt: Q96PV6)

PDB	External Database	Details
Gly 547	-	expression tag
Pro 548	-	expression tag
Asp 549	-	expression tag
Gly 801	-	linker
Ser 802	-	linker

C: 803 - 819 (UniProt: Q14684)
D: 1 - 399 (UniProt: Q5JVF3)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	135009.1
All*	Total formula weight	135009.1

*Water molecules are not included.