9T5N

Structure of heteromeric mouse LRRC8A/D Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1, Conformation 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Volume-regulated anion channel subunit LRRC8A	polymer	818	95127.4	4	UniProt (Q80WG5)	Mus musculus (house mouse)	Leucine-rich repeat-containing protein 8A,Protein ebouriffe,ebo,Swelling protein 1
2	E, F, G, H (G, H, I, J)	Sb1	polymer	118	13115.5	4		synthetic construct
3	I, J (E, F)	Volume-regulated anion channel subunit LRRC8D	polymer	924	105429.8	2	UniProt (Q8BGR2)	Mus musculus (house mouse)	Leucine-rich repeat-containing protein 5,Leucine-rich repeat-containing protein 8D

Sequence modifications

A, B, C, D: 2 - 810 (UniProt: Q80WG5)

PDB	External Database	Details
Met 0	-	initiating methionine
Ser 1	-	expression tag
Ala 811	-	expression tag
Leu 812	-	expression tag
Glu 813	-	expression tag
Val 814	-	expression tag
Leu 815	-	expression tag
Phe 816	-	expression tag
Gln 817	-	expression tag

E, F: 2 - 858 (UniProt: Q8BGR2)

PDB	External Database	Details
Met 0	-	initiating methionine
Ser 1	-	expression tag
Val 61	Ile 75	conflict
Val 63	Ala 128	conflict
Val 313	Leu 314	conflict
Ile 347	Thr 348	conflict
Ala 859	-	expression tag
Leu 860	-	expression tag
Glu 861	-	expression tag
Val 862	-	expression tag
Leu 863	-	expression tag
Phe 864	-	expression tag
Gln 865	-	expression tag
Gly 866	-	expression tag
Pro 867	-	expression tag
Gln 868	-	expression tag
Gly 869	-	expression tag
Thr 870	-	expression tag
Glu 871	-	expression tag
Gln 872	-	expression tag
Lys 873	-	expression tag
Leu 874	-	expression tag
Ile 875	-	expression tag
Ser 876	-	expression tag
Glu 877	-	expression tag
Glu 878	-	expression tag
Asp 879	-	expression tag
Leu 880	-	expression tag
Arg 881	-	expression tag
Gly 882	-	expression tag
Ala 883	-	expression tag
Ser 884	-	expression tag
Met 885	-	expression tag
Asp 886	-	expression tag
Glu 887	-	expression tag
Lys 888	-	expression tag
Thr 889	-	expression tag
Thr 890	-	expression tag
Gly 891	-	expression tag
Trp 892	-	expression tag
Arg 893	-	expression tag
Gly 894	-	expression tag
Gly 895	-	expression tag
His 896	-	expression tag
Val 897	-	expression tag
Val 898	-	expression tag
Glu 899	-	expression tag
Gly 900	-	expression tag
Leu 901	-	expression tag
Ala 902	-	expression tag
Gly 903	-	expression tag
Glu 904	-	expression tag
Leu 905	-	expression tag
Glu 906	-	expression tag
Gln 907	-	expression tag
Leu 908	-	expression tag
Arg 909	-	expression tag
Ala 910	-	expression tag
Arg 911	-	expression tag
Leu 912	-	expression tag
Glu 913	-	expression tag
His 914	-	expression tag
His 915	-	expression tag
Pro 916	-	expression tag
Gln 917	-	expression tag
Gly 918	-	expression tag
Gln 919	-	expression tag
Arg 920	-	expression tag
Glu 921	-	expression tag
Pro 922	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	643831.3
All*	Total formula weight	643831.3

*Water molecules are not included.