9T3H

Cryo-EM structure of a human KEOPS sub-complex bound to tRNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	EKC/KEOPS complex subunit TPRKB	polymer	188	21364.7	1	UniProt (Q9Y3C4)	Homo sapiens (human)	PRPK-binding protein,TP53RK-binding protein
2	B (B)	EKC/KEOPS complex subunit TP53RK	polymer	266	29885.3	1	UniProt (Q96S44)	Homo sapiens (human)	Atypical serine/threonine protein kinase TP53RK,Nori-2,TP53-regulating kinase,p53-related protein kinase
3	C (C)	Probable tRNA N6-adenosine threonylcarbamoyltransferase	polymer	335	36466.8	1	UniProt (Q9NPF4)	Homo sapiens (human)	N6-L-threonylcarbamoyladenine synthase,t(6)A synthase,O-sialoglycoprotein endopeptidase,hOSGEP,t(6)A37 threonylcarbamoyladenosine biosynthesis protein OSGEP,tRNA threonylcarbamoyladenosine biosynthesis protein OSGEP
4	D (F)	tRNA Ile AAU	polymer	77	24846.7	1		Homo sapiens

Sequence modifications

A: 2 - 175 (UniProt: Q9Y3C4)

PDB	External Database	Details
Met -12	-	initiating methionine
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag

B: 2 - 253 (UniProt: Q96S44)

PDB	External Database	Details
Met -12	-	initiating methionine
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	112563.5
All*	Total formula weight	112563.5

*Water molecules are not included.