9SQV

Cryo-EM structure of the ARISCdC(E33A):K63-Ub4 complex (Composite map)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (G, H)	Ubiquitin	polymer	76	8576.8	2	UniProt (P0CG48)	Homo sapiens (human)	Polyubiquitin-C
2	C, D (C, D)	BRCA1-A complex subunit Abraxas 1	polymer	302	34013.4	2	UniProt (Q6UWZ7)	Homo sapiens (human)	Coiled-coil domain-containing protein 98,Protein FAM175A
3	E, F (A, B)	Lys-63-specific deubiquitinase BRCC36	polymer	316	36061.9	2	UniProt (P46736)	Homo sapiens (human)	BRCA1-A complex subunit BRCC36,BRCA1/BRCA2-containing complex subunit 3,BRCA1/BRCA2-containing complex subunit 36,BRISC complex subunit BRCC36
4	G, H (F, E)	BRISC and BRCA1-A complex member 2	polymer	385	43721.6	2	UniProt (Q9NXR7)	Homo sapiens (human)	BRCA1-A complex subunit BRE,BRCA1/BRCA2-containing complex subunit 45,Brain and reproductive organ-expressed protein
5	I, J (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)

Sequence modifications

C, D: 1 - 265 (UniProt: Q6UWZ7)

PDB	External Database	Details
Met -36	-	initiating methionine
Trp -35	-	expression tag
Ser -34	-	expression tag
His -33	-	expression tag
Pro -32	-	expression tag
Gln -31	-	expression tag
Phe -30	-	expression tag
Glu -29	-	expression tag
Lys -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Trp -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Pro -12	-	expression tag
Gln -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Lys -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag

A, B: 1 - 316 (UniProt: P46736)

PDB	External Database	Details
Ala 33	Glu 33	engineered mutation

F, E: 1 - 383 (UniProt: Q9NXR7)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	244747.5
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	244878.3

*Water molecules are not included.