9SQ9

Crystal Structure of the MurT/GatD Enzyme Complex from Streptococcus pyogenes

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT	polymer	438	49037.6	2	UniProt (A0A8B6J667)	Streptococcus pyogenes MGAS10270
2	B, D (C, D)	Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD	polymer	263	29610.0	2	UniProt (A0A5S4TG60)	Streptococcus pyogenes MGAS10270	Lipid II isoglutaminyl synthase glutaminase subunit
3	E, I (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	F, H, J, O (A, C, B, D)	CITRIC ACID	non-polymer		192.1	4	Chemie (CIT)
5	G, K, L, M (A, B)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	4	Chemie (TRS)
6	N, P (B, D)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
7	Q, R, S, T (A, C, B, D)	water	water		18.0	707	Chemie (HOH)

Sequence modifications

A, B: 4 - 416 (UniProt: A0A8B6J667)

PDB	External Database	Details
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Pro -15	-	expression tag
Met -14	-	expression tag
Ser -13	-	expression tag
Asp -12	-	expression tag
Tyr -11	-	expression tag
Asp -10	-	expression tag
Ile -9	-	expression tag
Pro -8	-	expression tag
Thr -7	-	expression tag
Thr -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag
Ala 2	-	expression tag
Met 3	-	expression tag
Arg 184	His 213	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	157295.3
Non-Polymers*	Number of molecules	12
	Total formula weight	1600.1
All*	Total formula weight	158895.4

*Water molecules are not included.