9SQ9
Crystal Structure of the MurT/GatD Enzyme Complex from Streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-29 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.999998 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.280, 101.650, 178.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.140 - 2.050 |
| R-factor | 0.1752 |
| Rwork | 0.174 |
| R-free | 0.21810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.216 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.150 | 2.123 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.143 | 1.747 |
| Rmeas | 0.153 | |
| Rpim | 0.050 | 0.637 |
| Number of reflections | 91872 | 9310 |
| <I/σ(I)> | 9 | 1.18 |
| Completeness [%] | 90.6 | 93.08 |
| Redundancy | 7.4 | 7.1 |
| CC(1/2) | 0.997 | 0.557 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 24 % (w/v) PEG 3,350 200 mM tri-ammonium citrate pH 7.0 |
