9SC9

Crystal structure of Methanocaldococcus jannaschii Malate dehydrogenase C7GP mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	L-2-hydroxycarboxylate dehydrogenase (NAD(P)(+))	polymer	319	35054.6	4	UniProt (Q60176) Pfam (PF00056) Pfam (PF02866)	Methanocaldococcus jannaschii	MdhII
2	E, H, K, N (A, B, C, D)	NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		745.4	4	Chemie (NDP)
3	F, I, L, O (A, B, C, D)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	G, J, M, P (A, B, C, D)	POTASSIUM ION	non-polymer		39.1	4	Chemie (K)

Sequence modifications

A, B, C, D: 1 - 313 (UniProt: Q60176)

PDB	External Database	Details
Ser 24	Pro 24	engineered mutation
Lys 42	Glu 42	engineered mutation
Met 46	Glu 46	engineered mutation
Gly 56	Arg 56	engineered mutation
Ala 85	Pro 85	engineered mutation
Asn 105	Lys 105	engineered mutation
Glu 135	Asp 135	engineered mutation
Gly 137	Lys 137	engineered mutation
Lys 140	Arg 140	engineered mutation
Arg 198	Lys 198	engineered mutation
Gly 216	Thr 216	engineered mutation
Tyr 217	Lys 217	engineered mutation
Lys 219	Glu 219	engineered mutation
Asp 223	Arg 223	engineered mutation
Gly 266	Asp 266	engineered mutation
Lys 277	Arg 277	engineered mutation
Asn 289	Asp 289	engineered mutation
Gly 295	Ala 295	engineered mutation
Gly 305	Lys 305	engineered mutation
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	140218.2
Non-Polymers*	Number of molecules	12
	Total formula weight	3506.5
All*	Total formula weight	143724.7

*Water molecules are not included.