9SC9
Crystal structure of Methanocaldococcus jannaschii Malate dehydrogenase C7GP mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM07 |
| Synchrotron site | ESRF |
| Beamline | BM07 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.992, 75.534, 118.502 |
| Unit cell angles | 90.00, 95.57, 90.00 |
Refinement procedure
| Resolution | 117.940 - 2.900 |
| R-factor | 0.2068 |
| Rwork | 0.205 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.576 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.940 | 2.920 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.214 | 2.500 |
| Rmeas | 0.231 | 2.700 |
| Rpim | 0.091 | 1.218 |
| Number of reflections | 30234 | 2216 |
| <I/σ(I)> | 8.74 | 0.85 |
| Completeness [%] | 98.6 | |
| Redundancy | 6.85 | |
| CC(1/2) | 0.994 | 0.424 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 0.2M potassium sodium tartrate, 0.1M BisTris propane pH 7.5, 16-18% PEG 3350 Cryo-protectant: 20% Glycerol |
