9S6S
Ternary cryo-EM structure of human ALG9 with Dol25-PP-GlcNAc2Man6, Dol25-P-Man and Fab
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Alpha-1,2-mannosyltransferase ALG9 | polymer | 617 | 70347.5 | 1 | UniProt (Q9H6U8) | Homo sapiens (human) | Asparagine-linked glycosylation protein 9 homolog,Disrupted in bipolar disorder protein 1,Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase,Dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1 |
| 2 | B (H) | Hs9-8 Fab heavy chain | polymer | 240 | 25638.4 | 1 | synthetic construct | ||
| 3 | C (L) | Hs9-8 Fab light chain | polymer | 215 | 23258.8 | 1 | synthetic construct | ||
| 4 | D (B) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-1-O-phosphono-alpha-D-glucopyranose | branched | 1315.1 | 1 | In PDB | |||
| 5 | E, F (A) | [(3S,6Z,10E,14E)-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraenyl] dihydrogen phosphate | non-polymer | 440.6 | 2 | Chemie (A1JMT) PubChem (92854264) | |||
| 6 | G, I (A) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 2 | Chemie (Y01) PubChem (65082) | |||
| 7 | H (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) PubChem (24139) | |||
| 8 | J (A) | water | water | 18.0 | 22 | Chemie (HOH) |
Sequence modifications
A: 1 - 611 (UniProt: Q9H6U8)
| PDB | External Database | Details |
|---|---|---|
| Gly -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Thr 0 | - | expression tag |
| Ala 82 | Asp 82 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 119244.7 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 1315.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 2075.9 | |
| All* | Total formula weight | 122635.6 |
