9S6S
Ternary cryo-EM structure of human ALG9 with Dol25-PP-GlcNAc2Man6, Dol25-P-Man and Fab
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Alpha-1,2-mannosyltransferase ALG9 | polymer | 617 | 70347.5 | 1 | UniProt (Q9H6U8) | Homo sapiens (human) | Asparagine-linked glycosylation protein 9 homolog,Disrupted in bipolar disorder protein 1,Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase,Dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1 |
| 2 | B (H) | Hs9-8 Fab heavy chain | polymer | 240 | 25638.4 | 1 | synthetic construct | ||
| 3 | C (L) | Hs9-8 Fab light chain | polymer | 215 | 23258.8 | 1 | synthetic construct | ||
| 4 | D (A) | [(2~{R},3~{R},4~{R},5~{S},6~{R})-3-acetamido-5-[(2~{S},3~{R},4~{R},5~{S},6~{R})-3-acetamido-6-(hydroxymethyl)-5-[(2~{S},3~{S},4~{S},5~{R},6~{R})-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-4,5-bis(oxidanyl)oxan-2-yl]oxy-6-[[(2~{S},3~{S},4~{S},5~{R},6~{R})-6-(hydroxymethyl)-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxymethyl]-3,5-bis(oxidanyl)oxan-2-yl]oxy-4-oxidanyl-oxan-2-yl]oxy-6-(hydroxymethyl)-4-oxidanyl-oxan-2-yl] [oxidanyl-[(3~{S},6~{Z},10~{E},14~{E})-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraenoxy]phosphoryl] hydrogen phosphate | non-polymer | 1737.7 | 1 | Chemie (A1JL9) | |||
| 5 | E (A) | [(3S,6Z,10E,14E)-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraenyl] dihydrogen phosphate | non-polymer | 440.6 | 1 | Chemie (A1JMT) | |||
| 6 | F, H (A) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 2 | Chemie (Y01) | |||
| 7 | G (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 8 | I (A) | water | water | 18.0 | 22 | Chemie (HOH) |
Sequence modifications
A: 1 - 611 (UniProt: Q9H6U8)
| PDB | External Database | Details |
|---|---|---|
| Gly -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Thr 0 | - | expression tag |
| Ala 82 | Asp 82 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 119244.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 3372.9 | |
| All* | Total formula weight | 122617.6 |
