9S3F

Cryo-EM structure of Gephyrin in complex with Darpin 27F3, revealing linker-E domain interactions

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Isoform 5 of Gephyrin	polymer	754	81723.2	2	UniProt (Q03555)	Rattus norvegicus (Norway rat)	Putative glycine receptor-tubulin linker protein
2	C (C)	Darpin 27F3	polymer	182	19777.1	1		synthetic construct

Sequence modifications

A, B: 1 - 736 (UniProt: Q03555)

PDB	External Database	Details
Met -17	-	initiating methionine
Ala -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	183223.5
All*	Total formula weight	183223.5

*Water molecules are not included.