9S2F

Human debranched intron spliceosome (DIS)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (35)	Probable ATP-dependent RNA helicase DHX35	polymer	703	79012.2	1	UniProt (Q9H5Z1)	Homo sapiens (human)	DEAH box protein 35
2	B (5)	U5 snRNA	polymer	116	36908.7	1	GenBank (36515)	Homo sapiens (human)
3	C (A)	Pre-mRNA-processing-splicing factor 8	polymer	2335	273974.3	1	UniProt (Q6P2Q9)	Homo sapiens (human)	220 kDa U5 snRNP-specific protein,PRP8 homolog,Splicing factor Prp8,p220
4	D (B)	U5 small nuclear ribonucleoprotein 200 kDa helicase	polymer	2136	244823.4	1	UniProt (O75643)	Homo sapiens (human)	Activating signal cointegrator 1 complex subunit 3-like 1,BRR2 homolog,U5 snRNP-specific 200 kDa protein,U5-200KD
5	E (C)	116 kDa U5 small nuclear ribonucleoprotein component	polymer	972	109560.6	1	UniProt (Q15029)	Homo sapiens (human)	Elongation factor Tu GTP-binding domain-containing protein 2,SNU114 homolog,hSNU114,U5 snRNP-specific protein,116 kDa,U5-116 kDa
6	F (E)	U5 small nuclear ribonucleoprotein 40 kDa protein	polymer	357	39359.5	1	UniProt (Q96DI7)	Homo sapiens (human)	U5 snRNP 40 kDa protein,U5-40K,38 kDa-splicing factor,Prp8-binding protein,hPRP8BP,U5 snRNP-specific 40 kDa protein,WD repeat-containing protein 57
7	G (GP)	G patch domain-containing protein 1	polymer	931	103514.3	1	UniProt (Q9BRR8)	Homo sapiens (human)	Evolutionarily conserved G-patch domain-containing protein
8	H (I)	Pre-mRNA-splicing factor SYF1	polymer	855	100148.7	1	UniProt (Q9HCS7)	Homo sapiens (human)	Protein HCNP,XPA-binding protein 2
9	I (J)	Crooked neck-like protein 1	polymer	848	100610.0	1	UniProt (Q9BZJ0)	Homo sapiens (human)	Crooked neck homolog,hCrn
10	J (K)	Pre-mRNA-splicing factor SPF27	polymer	225	26163.4	1	UniProt (O75934)	Homo sapiens (human)	Breast carcinoma-amplified sequence 2,DNA amplified in mammary carcinoma 1 protein,Spliceosome-associated protein SPF 27
11	K (L)	Cell division cycle 5-like protein	polymer	802	92406.9	1	UniProt (Q99459)	Homo sapiens (human)	Cdc5-like protein,Pombe cdc5-related protein
12	L (M)	Pre-mRNA-splicing factor SYF2	polymer	243	28780.5	1	UniProt (O95926)	Homo sapiens (human)	CCNDBP1-interactor,p29
13	M (N)	Protein BUD31 homolog	polymer	144	17032.8	1	UniProt (P41223)	Homo sapiens (human)	Protein EDG-2,Protein G10 homolog
14	N (O)	Pre-mRNA-splicing factor RBM22	polymer	420	46959.6	1	UniProt (Q9NW64)	Homo sapiens (human)	RNA-binding motif protein 22,Zinc finger CCCH domain-containing protein 16
15	O (P)	Spliceosome-associated protein CWC15 homolog	polymer	229	26674.4	1	UniProt (Q9P013)	Homo sapiens (human)
16	P (Q)	Intron-binding protein aquarius	polymer	1485	171502.5	1	UniProt (O60306)	Homo sapiens (human)	Intron-binding protein of 160 kDa,IBP160
17	Q (R)	SNW domain-containing protein 1	polymer	536	61770.6	1	UniProt (Q13573)	Homo sapiens (human)	Nuclear protein SkiP,Nuclear receptor coactivator NCoA-62,Ski-interacting protein
18	R (S)	Peptidyl-prolyl cis-trans isomerase-like 1	polymer	166	18257.8	1	UniProt (Q9Y3C6)	Homo sapiens (human)	PPIase,Rotamase PPIL1
19	S (T)	Pleiotropic regulator 1	polymer	514	57280.8	1	UniProt (O43660)	Homo sapiens (human)
20	T (W)	Pre-mRNA-processing factor 17	polymer	579	65612.2	1	UniProt (O60508)	Homo sapiens (human)	Cell division cycle 40 homolog,EH-binding protein 3,Ehb3,PRP17 homolog,hPRP17
21	U (WD)	WD repeat domain-containing protein 83	polymer	315	34383.7	1	UniProt (Q9BRX9)	Homo sapiens (human)	Mitogen-activated protein kinase organizer 1,MAPK organizer 1
22	V (Z)	Coiled-coil domain-containing protein 12	polymer	166	19223.8	1	UniProt (Q8WUD4)	Homo sapiens (human)
23	W (a)	Small nuclear ribonucleoprotein Sm D3	polymer	126	13940.3	1	UniProt (P62318)	Homo sapiens (human)	Sm-D3,snRNP core protein D3
24	X (b)	Small nuclear ribonucleoprotein-associated proteins B and B'	polymer	240	24642.1	1	UniProt (P14678)	Homo sapiens (human)	snRNP-B,Sm protein B/B',Sm-B/B',SmB/B'
25	Y (c)	Small nuclear ribonucleoprotein Sm D1	polymer	119	13310.7	1	UniProt (P62314)	Homo sapiens (human)	Sm-D1,Sm-D autoantigen,snRNP core protein D1
26	Z (d)	Small nuclear ribonucleoprotein Sm D2	polymer	118	13551.9	1	UniProt (P62316)	Homo sapiens (human)	Sm-D2,snRNP core protein D2
27	AA (e)	Small nuclear ribonucleoprotein E	polymer	92	10817.6	1	UniProt (P62304)	Homo sapiens (human)	snRNP-E,Sm protein E,Sm-E,SmE
28	BA (f)	Small nuclear ribonucleoprotein F	polymer	86	9734.2	1	UniProt (P62306)	Homo sapiens (human)	snRNP-F,Sm protein F,SmF
29	CA (g)	Small nuclear ribonucleoprotein G	polymer	76	8508.1	1	UniProt (P62308)	Homo sapiens (human)	snRNP-G,Sm protein G,Sm-G,SmG
30	DA, EA, FA, GA (q, r, s, t)	Pre-mRNA-processing factor 19	polymer	504	55245.5	4	UniProt (Q9UMS4)	Homo sapiens (human)	Nuclear matrix protein 200,PRP19/PSO4 homolog,hPso4,RING-type E3 ubiquitin transferase PRP19,Senescence evasion factor
31	HA (y)	Peptidyl-prolyl cis-trans isomerase E	polymer	301	33475.8	1	UniProt (Q9UNP9)	Homo sapiens (human)	PPIase E,Cyclophilin E,Cyclophilin-33,Rotamase E
32	IA (D)	Pre-mRNA-splicing factor ISY1 homolog	polymer	285	33046.3	1	UniProt (Q9ULR0)	Homo sapiens (human)
33	JA (IN)	Intron	polymer	10	3016.7	1		Homo sapiens
34	KA (6)	U6 snRNA	polymer	106	34098.3	1	GenBank (NR_004394.1)	Homo sapiens (human)
35	LA (A)	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	1	Chemie (IHP)
36	MA (C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
37	NA (C)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
38	OA, PA, QA (N)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	37
	Total formula weight	2173084.7
Non-Polymers*	Number of molecules	6
	Total formula weight	1403.7
All*	Total formula weight	2174488.5

*Water molecules are not included.