9RPP

MeCP2 MBD domain (S134C) in complex with hydroxymethylated CA repeat DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Methyl-CpG-binding protein 2	polymer	99	11243.7	1	UniProt (P51608) Pfam (PF01429)	Homo sapiens (human)	MeCp-2 protein,MeCp2
2	B (B)	DNA (5'-D(*TP*CP*TP*GP*CP*AP*CP*AP*(5HC)P*AP*CP*AP*CP*AP*AP*TP*TP*AP*TP*A)-3')	polymer	20	6076.0	1		synthetic construct
3	C (C)	DNA (5'-D(*AP*TP*AP*TP*AP*AP*TP*TP*GP*TP*GP*TP*GP*TP*GP*TP*GP*CP*AP*G)-3')	polymer	20	6219.0	1		synthetic construct
4	D, E, F (A, B, C)	water	water		18.0	82	Chemie (HOH)

Sequence modifications

A: 77 - 165 (UniProt: P51608)

PDB	External Database	Details
Met 76	-	initiating methionine
Cys 134	Ser 134	engineered mutation
Ala 166	-	expression tag
Ala 167	-	expression tag
Ala 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	23538.7
All*	Total formula weight	23538.7

*Water molecules are not included.