9RN6
Crystal structure of a protein mimic of SARS-CoV-2 spike's HR1 domain in complex with two nanobodies bound to different epitopes
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Spike protein S2',Chimeric protein mimic of SARS-CoV-2 Spike HR1 | polymer | 241 | 26577.7 | 1 | UniProt (P0DTC2) | Severe acute respiratory syndrome coronavirus 2 | |
| 2 | B (B) | Nanobody NB278 | polymer | 123 | 13597.0 | 1 | Vicugna pacos | ||
| 3 | C (C) | Nanobody NB184 | polymer | 138 | 15294.8 | 1 | Vicugna pacos |
Sequence modifications
A: 3 - 76 (UniProt: P0DTC2)
A: 157 - 230 (UniProt: P0DTC2)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Asp 2 | - | expression tag |
| Lys 14 | Gln 926 | engineered mutation |
| Glu 32 | Ala 944 | engineered mutation |
| Glu 39 | Val 951 | engineered mutation |
| Asp 46 | Ala 958 | engineered mutation |
| Arg 60 | Ala 972 | engineered mutation |
| Glu 64 | Val 976 | engineered mutation |
| Gly 75 | Val 987 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Pro 77 | - | linker |
| Ala 78 | - | linker |
| Lys 79 | - | linker |
| Asp 80 | - | linker |
| Leu 81 | - | linker |
| Arg 82 | - | linker |
| Ser 83 | - | linker |
| Asp 84 | - | linker |
| Ile 85 | - | linker |
| Asp 86 | - | linker |
| Asn 87 | - | linker |
| Leu 88 | - | linker |
| Glu 89 | - | linker |
| Ser 90 | - | linker |
| Lys 91 | - | linker |
| Ile 92 | - | linker |
| Ala 93 | - | linker |
| Gly 94 | - | linker |
| Phe 95 | - | linker |
| Asn 96 | - | linker |
| Ser 97 | - | linker |
| Ser 98 | - | linker |
| Leu 99 | - | linker |
| Gln 100 | - | linker |
| Lys 101 | - | linker |
| Val 102 | - | linker |
| Leu 103 | - | linker |
| Thr 104 | - | linker |
| Asn 105 | - | linker |
| Leu 106 | - | linker |
| Ala 107 | - | linker |
| Gln 108 | - | linker |
| Lys 109 | - | linker |
| Asn 110 | - | linker |
| Gln 111 | - | linker |
| Asn 112 | - | linker |
| Val 113 | - | linker |
| Glu 114 | - | linker |
| Asp 115 | - | linker |
| Lys 116 | - | linker |
| Leu 117 | - | linker |
| Lys 118 | - | linker |
| Gly 119 | - | linker |
| Leu 120 | - | linker |
| Glu 121 | - | linker |
| Ser 122 | - | linker |
| Arg 123 | - | linker |
| Thr 124 | - | linker |
| Ser 125 | - | linker |
| Ser 126 | - | linker |
| Leu 127 | - | linker |
| Glu 128 | - | linker |
| Lys 129 | - | linker |
| Gln 130 | - | linker |
| Ile 131 | - | linker |
| Lys 132 | - | linker |
| Gly 133 | - | linker |
| Ile 134 | - | linker |
| Ala 135 | - | linker |
| Ser 136 | - | linker |
| Asn 137 | - | linker |
| Phe 138 | - | linker |
| Gln 139 | - | linker |
| Asn 140 | - | linker |
| Glu 141 | - | linker |
| Ile 142 | - | linker |
| Leu 143 | - | linker |
| Lys 144 | - | linker |
| Gln 145 | - | linker |
| Arg 146 | - | linker |
| Glu 147 | - | linker |
| Tyr 148 | - | linker |
| Leu 149 | - | linker |
| Val 150 | - | linker |
| Asn 151 | - | linker |
| Lys 152 | - | linker |
| Gly 153 | - | linker |
| Ser 154 | - | linker |
| Gly 155 | - | linker |
| Asn 156 | - | linker |
| Glu 166 | Ala 924 | engineered mutation |
| Lys 184 | Ala 942 | engineered mutation |
| Lys 191 | Gln 949 | engineered mutation |
| Lys 198 | Ala 956 | engineered mutation |
| Lys 223 | Leu 981 | engineered mutation |
| Gly 231 | - | expression tag |
| Gly 232 | - | expression tag |
| Gly 233 | - | expression tag |
| Gly 234 | - | expression tag |
| Ser 235 | - | expression tag |
| His 236 | - | expression tag |
| His 237 | - | expression tag |
| His 238 | - | expression tag |
| His 239 | - | expression tag |
| His 240 | - | expression tag |
| His 241 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 55469.4 | |
| All* | Total formula weight | 55469.4 |
