9R95
Cryo-EM structure of the human mitochondrial RNA polymerase transcription initiation complex (POLRMT/TFAM/TFB2M/DNA/RNA) with a slipped 3-mer RNA, pppGpGpA (slipped IC3)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase, mitochondrial | polymer | 1188 | 134632.3 | 1 | UniProt (O00411) Pfam (PF14700) Pfam (PF00940) | Homo sapiens (human) | MtRPOL |
| 2 | B (B) | Dimethyladenosine transferase 2, mitochondrial | polymer | 337 | 38901.3 | 1 | UniProt (Q9H5Q4) Pfam (PF00398) | Homo sapiens (human) | Hepatitis C virus NS5A-transactivated protein 5,HCV NS5A-transactivated protein 5,Mitochondrial 12S rRNA dimethylase 2,Mitochondrial transcription factor B2,h-mtTFB,h-mtTFB2,hTFB2M,mtTFB2,S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase 2 |
| 3 | C (C) | Transcription factor A, mitochondrial | polymer | 230 | 27637.6 | 1 | UniProt (Q00059) Pfam (PF00505) Pfam (PF09011) | Homo sapiens (human) | mtTFA,Mitochondrial transcription factor 1,MtTF1,Transcription factor 6,TCF-6,Transcription factor 6-like 2 |
| 4 | D (R) | RNA (5'-D(*(GTP))-R(P*GP*A)-3') | polymer | 3 | 1134.6 | 1 | synthetic construct | ||
| 5 | E (N) | Non-template strand DNA (56-MER) | polymer | 56 | 17486.3 | 1 | synthetic construct | ||
| 6 | F (T) | Template strand DNA (56-MER) | polymer | 56 | 17023.0 | 1 | synthetic construct |
Sequence modifications
C: 43 - 245 (UniProt: Q00059)
| PDB | External Database | Details |
|---|---|---|
| Met 16 | - | initiating methionine |
| Ser 17 | - | expression tag |
| Tyr 18 | - | expression tag |
| Tyr 19 | - | expression tag |
| His 20 | - | expression tag |
| His 21 | - | expression tag |
| His 22 | - | expression tag |
| His 23 | - | expression tag |
| His 24 | - | expression tag |
| His 25 | - | expression tag |
| Asp 26 | - | expression tag |
| Tyr 27 | - | expression tag |
| Asp 28 | - | expression tag |
| Ile 29 | - | expression tag |
| Pro 30 | - | expression tag |
| Thr 31 | - | expression tag |
| Thr 32 | - | expression tag |
| Glu 33 | - | expression tag |
| Asn 34 | - | expression tag |
| Leu 35 | - | expression tag |
| Tyr 36 | - | expression tag |
| Phe 37 | - | expression tag |
| Gln 38 | - | expression tag |
| Gly 39 | - | expression tag |
| Ala 40 | - | expression tag |
| Met 41 | - | expression tag |
| Gly 42 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 236815.1 | |
| All* | Total formula weight | 236815.1 |
