9R95
Cryo-EM structure of the human mitochondrial RNA polymerase transcription initiation complex (POLRMT/TFAM/TFB2M/DNA/RNA) with a slipped 3-mer RNA, pppGpGpA (slipped IC3)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000175 | molecular_function | 3'-5'-RNA exonuclease activity |
| A | 0000428 | cellular_component | DNA-directed RNA polymerase complex |
| A | 0001018 | molecular_function | mitochondrial promoter sequence-specific DNA binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0003899 | molecular_function | DNA-directed RNA polymerase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0006264 | biological_process | mitochondrial DNA replication |
| A | 0006390 | biological_process | mitochondrial transcription |
| A | 0006391 | biological_process | transcription initiation at mitochondrial promoter |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016779 | molecular_function | nucleotidyltransferase activity |
| A | 0032991 | cellular_component | protein-containing complex |
| A | 0034062 | molecular_function | 5'-3' RNA polymerase activity |
| A | 0034245 | cellular_component | mitochondrial DNA-directed RNA polymerase complex |
| A | 0042645 | cellular_component | mitochondrial nucleoid |
| A | 0043565 | molecular_function | sequence-specific DNA binding |
Functional Information from PROSITE/UniProt
| site_id | PS00489 |
| Number of Residues | 15 |
| Details | RNA_POL_PHAGE_2 Bacteriophage-type RNA polymerase family active site signature 2. ItRkvvKqtVMTvvY |
| Chain | Residue | Details |
| A | ILE985-TYR999 |
| site_id | PS00900 |
| Number of Residues | 12 |
| Details | RNA_POL_PHAGE_1 Bacteriophage-type RNA polymerase family active site signature 1. PVhqDGSCNGLQ |
| Chain | Residue | Details |
| A | PRO918-GLN929 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 428 |
| Details | Region: {"description":"Mediates interaction with TEFM","evidences":[{"source":"PubMed","id":"21278163","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 3 |
| Details | Active site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Region: {"description":"DNA-binding","evidences":[{"source":"PubMed","id":"29149603","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6ERP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6ERQ","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 3 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01026","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 68 |
| Details | DNA binding: {"description":"HMG box 1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00267","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Site: {"description":"Intercalates between bases and promotes DNA bending"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"Phosphoserine; by PKA","evidences":[{"source":"PubMed","id":"23201127","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
