9R75
Transcription initiation T. thermophilus RNA polymerase complex with anti-scrunched TC DNA template
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 315 | 35056.2 | 2 | UniProt (Q5SHR6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Thermus thermophilus HB8 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1119 | 125436.5 | 1 | UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Thermus thermophilus HB8 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1524 | 170997.4 | 1 | UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Thermus thermophilus HB8 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 99 | 11533.3 | 1 | UniProt (Q8RQE7) Pfam (PF01192) | Thermus thermophilus HB8 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase sigma factor SigA | polymer | 449 | 51470.1 | 1 | UniProt (Q72L95) | Thermus thermophilus HB8 | |
| 6 | G (G) | DNA (31-MER) | polymer | 53 | 16375.5 | 1 | synthetic construct | ||
| 7 | H (H) | DNA (35-MER) | polymer | 53 | 16450.5 | 1 | synthetic construct | ||
| 8 | I, J (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 9 | K, L (D) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
F: 1 - 423 (UniProt: Q72L95)
| PDB | External Database | Details |
|---|---|---|
| Met -25 | - | initiating methionine |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Gly -16 | - | expression tag |
| Val -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Leu -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Thr -11 | - | expression tag |
| Glu -10 | - | expression tag |
| Asn -9 | - | expression tag |
| Leu -8 | - | expression tag |
| Tyr -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Ser -4 | - | expression tag |
| Asn -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 462375.7 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 179.4 | |
| All* | Total formula weight | 462555.2 |
