9R75
Transcription initiation T. thermophilus RNA polymerase complex with anti-scrunched TC DNA template
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.8 |
| Software | Topaz,RELION,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
