9QVV

Crystal structure of the 4CHRD domain of human chordin, mutant designed to abolish binding to sulphated glycosaminoglycans

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Chordin	polymer	503	53531.4	1	UniProt (Q9H2X0)	Homo sapiens (human)
2	B (B)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		732.7	1	In PDB GlyTouCan (G32152BH)
3	C (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG) PubChem (24139)
4	D, E, F (A)	MALONATE ION	non-polymer		102.0	3	Chemie (MLI) PubChem (9084)
5	G (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL) PubChem (312)
6	H (A)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

A: 168 - 654 (UniProt: Q9H2X0)

PDB	External Database	Details
Ala 161	-	expression tag
Leu 162	-	expression tag
Ala 163	-	expression tag
Ala 164	-	expression tag
Pro 165	-	expression tag
Leu 166	-	expression tag
Thr 167	-	expression tag
Ala 193	Arg 193	engineered mutation
Ala 239	Arg 239	engineered mutation
Ala 530	Arg 530	engineered mutation
Ala 566	His 566	engineered mutation
Leu 630	Met 630	variant
Leu 653	-	insertion
Pro 655	-	expression tag
Arg 656	-	expression tag
Gly 657	-	expression tag
Ser 658	-	expression tag
His 659	-	expression tag
His 660	-	expression tag
His 661	-	expression tag
His 662	-	expression tag
His 663	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	53531.4
Branched	Number of molecules	1
	Total formula weight	732.7
Non-Polymers*	Number of molecules	5
	Total formula weight	562.8
All*	Total formula weight	54826.9

*Water molecules are not included.