9QVV

Crystal structure of the 4CHRD domain of human chordin, mutant designed to abolish binding to sulphated glycosaminoglycans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	99
Detector technology	PIXEL
Collection date	2025-03-30
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	I 2 2 2
Unit cell lengths	120.142, 123.800, 123.787
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.890 - 2.720
R-factor	0.2419
Rwork	0.237
R-free	0.27940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.616
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.890	2.850
High resolution limit [Å]	2.720	2.720
Rmerge	0.090	2.195
Rmeas	0.094
Rpim	0.035
Number of reflections	24327	2912
<I/σ(I)>	13.8	1
Completeness [%]	96.7	89.2
Redundancy	13.5
CC(1/2)	0.999	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277.15	0.1M Bis Tris propane pH 8.5, 0.2M Sodium malonate dibasic monohydrate, 20% PEG 3350 PACT condition H12