9QT9

Cryo-EM structure of the core of the Arabidopsis thaliana UBR4/DI19/CALM1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	Auxin transport protein (BIG)	polymer	5141	572672.3	2	UniProt (A0A1I9LSK4) Pfam (PF00569) Pfam (PF24079) Pfam (PF13764) UniProt (by SIFTS) (Q9SRU2)	Arabidopsis thaliana (thale cress)
2	B, E (E, F)	Calmodulin-1	polymer	177	20042.9	2	UniProt (P0DH95) Pfam (PF13499)	Arabidopsis thaliana (thale cress)	CaM-1
3	C, F (C, D)	Protein DEHYDRATION-INDUCED 19	polymer	221	24828.0	2	UniProt (Q39083) Pfam (PF05605) Pfam (PF14571)	Arabidopsis thaliana (thale cress)	AtDi19-1

Sequence modifications

A, B: 22 - 5098 (UniProt: A0A1I9LSK4)

PDB	External Database	Details
Gly 5099	-	expression tag
Ser 5100	-	expression tag
Leu 5101	-	expression tag
Glu 5102	-	expression tag
Val 5103	-	expression tag
Leu 5104	-	expression tag
Phe 5105	-	expression tag
Gln 5106	-	expression tag
Gly 5107	-	expression tag
Pro 5108	-	expression tag
Ala 5109	-	expression tag
Glu 5110	-	expression tag
Ala 5111	-	expression tag
Ala 5112	-	expression tag
Ala 5113	-	expression tag
Lys 5114	-	expression tag
Glu 5115	-	expression tag
Ala 5116	-	expression tag
Ala 5117	-	expression tag
Ala 5118	-	expression tag
Lys 5119	-	expression tag
Glu 5120	-	expression tag
Ala 5121	-	expression tag
Ala 5122	-	expression tag
Ala 5123	-	expression tag
Lys 5124	-	expression tag
Glu 5125	-	expression tag
Ala 5126	-	expression tag
Ala 5127	-	expression tag
Ala 5128	-	expression tag
Lys 5129	-	expression tag
Ala 5130	-	expression tag
Leu 5131	-	expression tag
Glu 5132	-	expression tag
Ala 5133	-	expression tag
Glu 5134	-	expression tag
Ala 5135	-	expression tag
Ala 5136	-	expression tag
Ala 5137	-	expression tag
Lys 5138	-	expression tag
Glu 5139	-	expression tag
Ala 5140	-	expression tag
Ala 5141	-	expression tag
Ala 5142	-	expression tag
Lys 5143	-	expression tag
Glu 5144	-	expression tag
Ala 5145	-	expression tag
Ala 5146	-	expression tag
Ala 5147	-	expression tag
Lys 5148	-	expression tag
Glu 5149	-	expression tag
Ala 5150	-	expression tag
Ala 5151	-	expression tag
Ala 5152	-	expression tag
Lys 5153	-	expression tag
Ala 5154	-	expression tag
His 5155	-	expression tag
His 5156	-	expression tag
His 5157	-	expression tag
His 5158	-	expression tag
His 5159	-	expression tag
His 5160	-	expression tag
His 5161	-	expression tag
His 5162	-	expression tag

E, F: 1 - 149 (UniProt: P0DH95)

PDB	External Database	Details
Met -27	-	initiating methionine
Asp -26	-	expression tag
Tyr -25	-	expression tag
Lys -24	-	expression tag
Asp -23	-	expression tag
His -22	-	expression tag
Asp -21	-	expression tag
Gly -20	-	expression tag
Asp -19	-	expression tag
Tyr -18	-	expression tag
Lys -17	-	expression tag
Asp -16	-	expression tag
His -15	-	expression tag
Asp -14	-	expression tag
Ile -13	-	expression tag
Asp -12	-	expression tag
Tyr -11	-	expression tag
Lys -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Lys -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag

C, D: 1 - 206 (UniProt: Q39083)

PDB	External Database	Details
Gly 207	-	expression tag
Gly 208	-	expression tag
Ser 209	-	expression tag
Gly 210	-	expression tag
Gly 211	-	expression tag
Ser 212	-	expression tag
Ala 213	-	expression tag
Trp 214	-	expression tag
Ser 215	-	expression tag
His 216	-	expression tag
Pro 217	-	expression tag
Gln 218	-	expression tag
Phe 219	-	expression tag
Glu 220	-	expression tag
Lys 221	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	1235086.4
All*	Total formula weight	1235086.4

*Water molecules are not included.