9QS0

Crystal Structure of the MAR1-FUS1 Gamete Adhesion Complex in Chlamydomonas reinhardtii

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	FUS1p	polymer	470	51590.7	2	UniProt (Q39594)	Chlamydomonas reinhardtii	Fus1 protein
2	C, D (C, D)	Minus adhesion receptor 1	polymer	171	17531.1	2	UniProt (A0A0U2YF84)	Chlamydomonas reinhardtii
3	E, F (E, F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	G, I, L, N, O... (A, B, C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	9	Chemie (NAG) PubChem (24139)
5	H, J, K, M, P... (A, B, C, D)	GLYCEROL	non-polymer		92.1	9	Chemie (GOL) PubChem (753)
6	Y, Z (A, B)	water	water		18.0	6	Chemie (HOH)

Sequence modifications

A, B: 17 - 479 (UniProt: Q39594)

PDB	External Database	Details
Gly 480	-	expression tag
His 481	-	expression tag
His 482	-	expression tag
His 483	-	expression tag
His 484	-	expression tag
His 485	-	expression tag
His 486	-	expression tag

C, D: 26 - 161 (UniProt: A0A0U2YF84)

PDB	External Database	Details
Gln 56	Asn 56	engineered mutation
Gln 64	Asn 64	engineered mutation
Gln 72	Asn 72	engineered mutation
Gln 75	Asn 75	engineered mutation
Asp 162	-	expression tag
Asp 163	-	expression tag
Asp 164	-	expression tag
Asp 165	-	expression tag
Lys 166	-	expression tag
Ala 167	-	expression tag
Gly 168	-	expression tag
Trp 169	-	expression tag
Ser 170	-	expression tag
His 171	-	expression tag
Pro 172	-	expression tag
Gln 173	-	expression tag
Phe 174	-	expression tag
Glu 175	-	expression tag
Lys 176	-	expression tag
Gly 177	-	expression tag
Gly 178	-	expression tag
Gly 179	-	expression tag
Ser 180	-	expression tag
Gly 181	-	expression tag
Gly 182	-	expression tag
Gly 183	-	expression tag
Ser 184	-	expression tag
Gly 185	-	expression tag
Gly 186	-	expression tag
Gly 187	-	expression tag
Ser 188	-	expression tag
Trp 189	-	expression tag
Ser 190	-	expression tag
His 191	-	expression tag
Pro 192	-	expression tag
Gln 193	-	expression tag
Phe 194	-	expression tag
Glu 195	-	expression tag
Lys 196	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	138243.6
Branched	Number of molecules	2
	Total formula weight	848.8
Non-Polymers*	Number of molecules	18
	Total formula weight	2819.7
All*	Total formula weight	141912.2

*Water molecules are not included.