9QS0

Crystal Structure of the MAR1-FUS1 Gamete Adhesion Complex in Chlamydomonas reinhardtii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980095922947
Spacegroup name	C 1 2 1
Unit cell lengths	348.395, 89.684, 97.327
Unit cell angles	90.00, 98.66, 90.00

Refinement procedure

Resolution	45.240 - 3.100
R-factor	0.2179
Rwork	0.216
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.614
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.240	3.180
High resolution limit [Å]	3.100	3.100
Number of reflections	87495	13154
<I/σ(I)>	2.42
Completeness [%]	73.6
Redundancy	3.4
CC(1/2)	0.937	0.156

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris-HCl pH 8.5, 1 uM Anderson-Evans polyoxotungstate