9QEE
Cryo-EM structure of a DNA-bound XPF-ERCC1-SLX4(330-555)-SLX4IP complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | DNA excision repair protein ERCC-1 | polymer | 297 | 32598.3 | 1 | UniProt (P07992) Pfam (PF03834) Pfam (PF00633) | Homo sapiens (human) | |
| 2 | B (C) | Protein SLX4IP | polymer | 408 | 45627.7 | 1 | UniProt (Q5VYV7) Pfam (PF15744) | Homo sapiens (human) | SLX4-interacting protein |
| 3 | C (U) | DNA strand 1 | polymer | 50 | 15425.9 | 1 | synthetic construct | ||
| 4 | D (V) | DNA strand 2 | polymer | 50 | 15585.0 | 1 | synthetic construct | ||
| 5 | E (D) | Structure-specific endonuclease subunit SLX4 | polymer | 271 | 29617.7 | 1 | UniProt (Q8IY92) | Homo sapiens (human) | BTB/POZ domain-containing protein 12 |
| 6 | F (A) | DNA repair endonuclease XPF | polymer | 935 | 106894.5 | 1 | UniProt (Q92889) Pfam (PF02732) | Homo sapiens (human) | DNA excision repair protein ERCC-4,DNA repair protein complementing XP-F cells,Xeroderma pigmentosum group F-complementing protein |
| 7 | G, H (U, A) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) |
Sequence modifications
D: 330 - 555 (UniProt: Q8IY92)
A: 1 - 916 (UniProt: Q92889)
| PDB | External Database | Details |
|---|---|---|
| Met 285 | - | initiating methionine |
| Ala 286 | - | expression tag |
| Ser 287 | - | expression tag |
| Trp 288 | - | expression tag |
| Ser 289 | - | expression tag |
| His 290 | - | expression tag |
| Pro 291 | - | expression tag |
| Gln 292 | - | expression tag |
| Phe 293 | - | expression tag |
| Glu 294 | - | expression tag |
| Lys 295 | - | expression tag |
| Gly 296 | - | expression tag |
| Gly 297 | - | expression tag |
| Gly 298 | - | expression tag |
| Ser 299 | - | expression tag |
| Gly 300 | - | expression tag |
| Gly 301 | - | expression tag |
| Gly 302 | - | expression tag |
| Ser 303 | - | expression tag |
| Gly 304 | - | expression tag |
| Gly 305 | - | expression tag |
| Gly 306 | - | expression tag |
| Ser 307 | - | expression tag |
| Trp 308 | - | expression tag |
| Ser 309 | - | expression tag |
| His 310 | - | expression tag |
| Pro 311 | - | expression tag |
| Gln 312 | - | expression tag |
| Phe 313 | - | expression tag |
| Glu 314 | - | expression tag |
| Lys 315 | - | expression tag |
| Ser 316 | - | expression tag |
| Gly 317 | - | expression tag |
| Gly 318 | - | expression tag |
| Gly 319 | - | expression tag |
| Ser 320 | - | expression tag |
| Glu 321 | - | expression tag |
| Asn 322 | - | expression tag |
| Leu 323 | - | expression tag |
| Tyr 324 | - | expression tag |
| Phe 325 | - | expression tag |
| Gln 326 | - | expression tag |
| Ser 327 | - | expression tag |
| Asn 328 | - | expression tag |
| Ala 329 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -18 | - | initiating methionine |
| Gly -17 | - | expression tag |
| Ser -16 | - | expression tag |
| Ser -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 245749.1 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 109.9 | |
| All* | Total formula weight | 245859.0 |
