9QD6
High resolution structure of the artificially maturated [FeFe]-hydrogenase from Nitratidesulfovibrio vulgaris str. Hildenborough
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Periplasmic [Fe] hydrogenase small subunit | polymer | 88 | 10082.4 | 1 | UniProt (P07603) Pfam (PF02256) | Nitratidesulfovibrio vulgaris str. Hildenborough | Fe hydrogenlyase small chain |
| 2 | B (A) | Periplasmic [Fe] hydrogenase large subunit | polymer | 405 | 44397.1 | 1 | UniProt (P07598) Pfam (PF00037) Pfam (PF02906) | Nitratidesulfovibrio vulgaris str. Hildenborough | Fe hydrogenlyase |
| 3 | C (A) | dicarbonyl[bis(cyanide-kappaC)]-mu-(iminodimethanethiolatato-1kappaS:2kappaS)-mu-(oxomethylidene)diiron(2+) | non-polymer | 355.0 | 1 | Chemie (402) | |||
| 4 | D, E, F (A) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 3 | Chemie (SF4) | |||
| 5 | G (A) | TRIS(HYDROXYETHYL)AMINOMETHANE | non-polymer | 163.2 | 1 | Chemie (TAM) | |||
| 6 | H (A) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 1 | Chemie (PG4) | |||
| 7 | I, J (B, A) | water | water | 18.0 | 375 | Chemie (HOH) |
Sequence modifications
A: 1 - 397 (UniProt: P07598)
| PDB | External Database | Details |
|---|---|---|
| Trp 398 | - | expression tag |
| Ser 399 | - | expression tag |
| His 400 | - | expression tag |
| Pro 401 | - | expression tag |
| Gln 402 | - | expression tag |
| Phe 403 | - | expression tag |
| Glu 404 | - | expression tag |
| Lys 405 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 54479.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1767.3 | |
| All* | Total formula weight | 56246.8 |
