9Q7W
One of a series of engineered variants of I-OnuI meganuclease targeting altered DNA target sequence
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ribosomal protein 3/homing endonuclease-like protein fusion | polymer | 300 | 34375.7 | 1 | UniProt (Q4VWW5) Pfam (PF00961) | synthetic construct | |
| 2 | B (B) | DNA (26-MER) | polymer | 26 | 7974.1 | 1 | synthetic construct | ||
| 3 | C (C) | DNA (26-MER) | polymer | 26 | 7997.2 | 1 | synthetic construct | ||
| 4 | D, E (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 5 | F, G (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 6 | H, I (B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 7 | J, K, L (A, B, C) | water | water | 18.0 | 15 | Chemie (HOH) |
Sequence modifications
A: 5 - 301 (UniProt: Q4VWW5)
| PDB | External Database | Details |
|---|---|---|
| Met 2 | - | initiating methionine |
| Ala 3 | - | expression tag |
| Ser 4 | - | expression tag |
| Lys 108 | Met 520 | conflict |
| Ser 115 | Cys 527 | conflict |
| Glu 125 | Ile 537 | conflict |
| Asn 153 | Ile 565 | conflict |
| Ser 180 | Cys 592 | conflict |
| Ile 182 | Phe 594 | conflict |
| Arg 199 | Val 611 | conflict |
| Thr 201 | Ser 613 | conflict |
| Val 225 | Lys 637 | conflict |
| Ser 227 | Lys 639 | conflict |
| Thr 236 | Asp 648 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 50347.1 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 388.5 | |
| All* | Total formula weight | 50735.6 |
