9Q7U
Composite map for Cryo-EM structure of DNMT3A2-DNMT3B3 tetramer bound to 167H3K36me2-nucleosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | Histone H3 | polymer | 135 | 15331.0 | 2 | UniProt (A0A310TTQ1) Pfam (PF00125) | Xenopus laevis (African clawed frog) | |
| 2 | B, F (B, F) | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 3 | C, G (C, G) | Histone H2A type 1 | polymer | 129 | 13978.2 | 2 | UniProt (P06897) Pfam (PF00125) Pfam (PF16211) | Xenopus laevis (African clawed frog) | |
| 4 | D, H (D, H) | Histone H2B 1.1 | polymer | 123 | 13655.9 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 5 | I (I) | DNA (162-MER) | polymer | 167 | 51786.0 | 1 | Homo sapiens | ||
| 6 | J (J) | DNA (162-MER) | polymer | 167 | 51327.7 | 1 | Homo sapiens | ||
| 7 | K, L (V, Z) | Isoform 7 of DNA (cytosine-5)-methyltransferase 3B | polymer | 580 | 65568.4 | 2 | UniProt (Q9UBC3) Pfam (PF00855) Pfam (PF17980) Pfam (PF21255) Pfam (PF00145) | Homo sapiens (human) | Dnmt3b,DNA methyltransferase HsaIIIB,DNA MTase HsaIIIB,M.HsaIIIB |
| 8 | M, N (K, L) | DNA (cytosine-5)-methyltransferase 3A | polymer | 689 | 77914.7 | 2 | UniProt (Q9Y6K1) Pfam (PF00855) Pfam (PF17980) Pfam (PF21255) Pfam (PF00145) | Homo sapiens (human) | Dnmt3a,DNA methyltransferase HsaIIIA,M.HsaIIIA |
| 9 | O, P, Q, R, S... (V, K, L) | ZINC ION | non-polymer | 65.4 | 9 | Chemie (ZN) | |||
| 10 | U, Y (K, L) | 5'-S-[(3S)-3-azaniumyl-3-carboxypropyl]-5'-thioadenosine | non-polymer | 385.4 | 2 | Chemie (SAO) PubChem (56593148) |
Sequence modifications
C, G: 1 - 129 (UniProt: P06897)
D, H: 1 - 122 (UniProt: P02281)
V, Z: 191 - 770 (UniProt: Q9UBC3)
| PDB | External Database | Details |
|---|---|---|
| Arg 99 | Gly 100 | engineered mutation |
| Ser 123 | Ala 124 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Thr 29 | Ser 33 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Lys 391 | - | insertion |
| Asp 392 | - | insertion |
| Tyr 393 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 498799.0 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1359.5 | |
| All* | Total formula weight | 500158.5 |
