9Q79

Crystal structure of T. cruzi EIF4E6 in complex with EIF4G5 peptide and cap-4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, G (A, C, G, E)	Putative Eukaryotic translation initiation factor 4E type 6	polymer	186	21401.3	4	UniProt (A0A2V2VTW2)	Trypanosoma cruzi Dm28c
2	D, E, F, H (B, D, H, F)	Eukaryotic translation initiation factor 4 gamma 5	polymer	49	5706.4	4	UniProt (A0A2V2VHI6)	Trypanosoma cruzi
3	I, J (A, C)	2-amino-9-[(2R,3R,4S,5R)-5-({[(R)-{[(R)-{[(S)-({(2R,3R,4R,5R)-3-{[(R)-{[(2R,3R,4R,5R)-3-{[(S)-{[(2R,3R,4R,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3-{[(S)-hydroxy{[(2R,3R,4R,5R)-3-hydroxy-4-methoxy-5-(3-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methoxy}phosphoryl]oxy}-4-methoxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}-5-(6-amino-9H-purin-9-yl)-4-methoxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}-5-[6-(dimethylamino)-9H-purin-9-yl]-4-methoxytetrahydrofuran-2-yl}methoxy)(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}methyl)-3,4-dihydroxytetrahydrofuran-2-yl]-7-methyl-6-oxo-6,9-dihydro-3H-purin-7-ium	non-polymer		1826.2	2	Chemie (LRM)
4	K (E)	CITRATE ANION	non-polymer		189.1	1	Chemie (FLC)
5	L, M, N, O, P... (A, C, G, B, D...)	water	water		18.0	148	Chemie (HOH)

Sequence modifications

B, D, H, F: 71 - 112 (UniProt: A0A2V2VHI6)

PDB	External Database	Details
Met 70	-	initiating methionine
His 113	-	expression tag
His 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	108431.2
Non-Polymers*	Number of molecules	3
	Total formula weight	3841.5
All*	Total formula weight	112272.6

*Water molecules are not included.