9Q79

Crystal structure of T. cruzi EIF4E6 in complex with EIF4G5 peptide and cap-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	81.211, 93.236, 119.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.620 - 2.300
R-factor	0.2376
Rwork	0.234
R-free	0.30440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.650	2.390
High resolution limit [Å]	2.300	2.300
Number of reflections	30384	1520
<I/σ(I)>	8.6
Completeness [%]	96.1
Redundancy	13.5
CC(1/2)	0.998	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Complex at 7.3 mg/mL in 20 mM Tris-HCl, 150 mM NaCl, 2 mM DTT, pH 8.0 (pre-incubated with cap-4) with crystallization buffer containing 100 mM tri-sodium citrate (pH 3.5) and 20.6% w/v PEG 3350