9Q75

Crystal structure of T. cruzi EIF4E5 in complex with EIF4G1 peptide and cap-4.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, C, E)	Putative Eukaryotic translation initiation factor 4E type 5	polymer	200	22611.6	3	UniProt (A0A2V2XI07)	Trypanosoma cruzi Dm28c
2	D, E, F (B, D, F)	Eukaryotic translation initiation factor 4 gamma 1	polymer	48	5798.6	3	UniProt (A0A2V2UXK5)	Trypanosoma cruzi Dm28c
3	G, H, I, K, M (A, C, E)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
4	J, L (C, E)	2-amino-9-[(2R,3R,4S,5R)-5-({[(R)-{[(R)-{[(S)-({(2R,3R,4R,5R)-3-{[(R)-{[(2R,3R,4R,5R)-3-{[(S)-{[(2R,3R,4R,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3-{[(S)-hydroxy{[(2R,3R,4R,5R)-3-hydroxy-4-methoxy-5-(3-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methoxy}phosphoryl]oxy}-4-methoxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}-5-(6-amino-9H-purin-9-yl)-4-methoxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}-5-[6-(dimethylamino)-9H-purin-9-yl]-4-methoxytetrahydrofuran-2-yl}methoxy)(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}methyl)-3,4-dihydroxytetrahydrofuran-2-yl]-7-methyl-6-oxo-6,9-dihydro-3H-purin-7-ium	non-polymer		1826.2	2	Chemie (LRM)
5	N, O, P, Q, R... (A, C, E, B, D...)	water	water		18.0	572	Chemie (HOH)

Sequence modifications

A, C, E: 1 - 200 (UniProt: A0A2V2XI07)

PDB	External Database	Details
Lys 127	Thr 127	conflict

B, D, F: 108 - 147 (UniProt: A0A2V2UXK5)

PDB	External Database	Details
Met 106	-	initiating methionine
Gly 107	-	expression tag
His 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag
His 151	-	expression tag
His 152	-	expression tag
His 153	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	85230.8
Non-Polymers*	Number of molecules	7
	Total formula weight	4132.7
All*	Total formula weight	89363.4

*Water molecules are not included.