9Q75
Crystal structure of T. cruzi EIF4E5 in complex with EIF4G1 peptide and cap-4.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.380, 88.889, 110.165 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.210 - 1.770 |
| R-factor | 0.16052 |
| Rwork | 0.158 |
| R-free | 0.21501 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.506 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.180 | 1.900 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 67238 | 3362 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 95.8 | |
| Redundancy | 13.9 | |
| CC(1/2) | 0.999 | 0.773 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Complex at 16 mg/mL in 20 mM Tris-HCl, 150 mM NaCl, 2 mM DTT, pH 8.0 (pre-incubated with cap-4) with crystallization buffer containing 0.29 M (NH4)2SO4; 0.1 M Tris-HCl pH 8.5; 26% (m/V) PEG 3350 |
