9Q74

Crystal structure of T. brucei EIF4E5 in complex with EIF4G1 peptide and cap-4.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Eukaryotic translation initiation factor 4E type 5	polymer	195	22007.0	1	UniProt (Q38B99) Pfam (PF01652)	Trypanosoma brucei brucei TREU927
2	B (B)	MIF4G domain-containing protein	polymer	48	5551.4	1	UniProt (Q57VB4)	Trypanosoma brucei brucei TREU927
3	C (A)	2-amino-9-[(2R,3R,4S,5R)-5-({[(R)-{[(R)-{[(S)-({(2R,3R,4R,5R)-3-{[(R)-{[(2R,3R,4R,5R)-3-{[(S)-{[(2R,3R,4R,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3-{[(S)-hydroxy{[(2R,3R,4R,5R)-3-hydroxy-4-methoxy-5-(3-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methoxy}phosphoryl]oxy}-4-methoxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}-5-(6-amino-9H-purin-9-yl)-4-methoxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}-5-[6-(dimethylamino)-9H-purin-9-yl]-4-methoxytetrahydrofuran-2-yl}methoxy)(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}methyl)-3,4-dihydroxytetrahydrofuran-2-yl]-7-methyl-6-oxo-6,9-dihydro-3H-purin-7-ium	non-polymer		1826.2	1	Chemie (LRM)
4	D, E, F, G, H... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	10	Chemie (EDO)
5	N, O (A, B)	water	water		18.0	237	Chemie (HOH)

Sequence modifications

B: 116 - 155 (UniProt: Q57VB4)

PDB	External Database	Details
Met 114	-	initiating methionine
Gly 115	-	expression tag
His 156	-	expression tag
His 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	27558.4
Non-Polymers*	Number of molecules	11
	Total formula weight	2446.9
All*	Total formula weight	30005.3

*Water molecules are not included.